The aim of the following study is to identify and quantify microorganisms in the saliva of black stain patients using species-specific probes for Actinomyces israelii, Actinomyces naeslundii, Streptococcus mutans and Lactobacillus spp, as well as a universal probe for bacterial 16S rDNA, to obtain the figure of the total bacterial counts (for test and control group). This study represents a first phase of the research line on extrinsic tooth pigmentation on developmental age patients. Materials and methods. The selected sample is composed of 20 subjects aged between 2 and 15 years and in good general and oral health among the patients included in a dental examination trial at the Department of Pediatric Dentistry of the University Hospital of Palermo, after obteining informed consent from parents. Subjects with a positive anamnesis for systemic diseases or with enamel defects were excluded from the study. Exclusion criteria were also a diet including regular use of tea, coffee, iron supplements, or antibiotic therapies in the 3 months prior to enrollment and the use of mouthwashes/toothpastes containing antiseptics. Parents/tutors were asked to complete a questionnaire on their occupational status and level of education, as well as their eating habits, the frequency of brushing and the patient's dental records. A sample of 20 patients without BS was used as control group. From all subjects participating to the study a sample of 5ml of unstimulated saliva was recovered from the right and left buccal area through a sterile syringe, after 2 minutes waiting for the accumulation of an adequate amount of saliva. Statistical analysis was carried out using descriptive and inferential statistics (mean and standard deviation; chi-square test, t test for non-paired data). StatView 5.0.1 statistical software (SAS Institute, Cary, NC, USA) was used for this purpose, and the tests were considered significant at p values less than or equal to 0.05. The study protocol will be presented for approval to the Palermo 1 ethics committee.
|Titolo:||Study of oral microbiota and diet as casual factors of Black Stains in the pediatric patient|
|Data di pubblicazione:||2019|
|Citazione:||Cusimano, D. (2019). Study of oral microbiota and diet as casual factors of Black Stains in the pediatric patient. In O. Di Fede, & E. Fiorentino (a cura di), 1a Giornata delle Scuole di Specializzazione "Restate al Di.Chir.On.S." - Principali Linee di Ricerca delle Scuole di Specializzazione (pp. 51-52). Palermo : New Digital Frontiers srl.|
|Appare nelle tipologie:||2.08 Abstract in atti di convegno pubblicato in volume|