A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.

SETTANNI, L., VALMORRI, S., VAN SINDEREN, D., SUZZI, G., PAPARELLA, A., CORSETTI, A. (2006). Combination of multiplex PCR and PCR-DGGE for monitoring common sourdough-associated Lactobacillus species. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72, 3793-3796 [10.1128/AEM.72.5.3793-3796.2006].

Combination of multiplex PCR and PCR-DGGE for monitoring common sourdough-associated Lactobacillus species

SETTANNI, Luca;
2006-01-01

Abstract

A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.
2006
SETTANNI, L., VALMORRI, S., VAN SINDEREN, D., SUZZI, G., PAPARELLA, A., CORSETTI, A. (2006). Combination of multiplex PCR and PCR-DGGE for monitoring common sourdough-associated Lactobacillus species. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72, 3793-3796 [10.1128/AEM.72.5.3793-3796.2006].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/37449
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