It is now widely recognised that the cross-talk between cancer and stromal cells may play a crucial role in cancer progression. However little is known about the complex underlying molecular mechanisms that occur within the tumor microenvironment. Fibroblasts are the major stromal cells with multiple roles, especially towards both the extracellular matrix and the neighbouring cell population, including neoplastic cells. Consequently, proteomic analyses would provide a wider resource for a better understanding of the potential modulating effects exerted by fibroblasts on cancer cells. In this report we describe the effects of fibroblast stimulation on the breast cancer cell line (8701-BC) proteomics, using a trans-well co-culture system. Our results clearly indicate that fibroblasts induce considerable proteomic modulations on 8701-BC, mainly in the cytoskeleton proteins and glycolytic enzymes. Additionally, fibroblast-conditioned medium increased neoplastic cell proliferation and invasion with a concurrent up-regulation of the c-myc oncogene. Collectively these results suggest that fibroblast stimulation may enhance the malignant potential of breast cancer cells in vitro.

Cancemi, P., Albanese, N., DI CARA, G., Marabeti, M., Costantini, F., Minafra, S., et al. (2010). Multiple changes induced by fibroblasts on breast cancer cells. CONNECTIVE TISSUE RESEARCH, 51 [10.3109/03008200903100651].

Multiple changes induced by fibroblasts on breast cancer cells.

CANCEMI, Patrizia;ALBANESE, Nadia Ninfa;DI CARA, Gianluca;MARABETI, Maria Rita;COSTANTINI, Francesca;PUCCI, Ida
2010

Abstract

It is now widely recognised that the cross-talk between cancer and stromal cells may play a crucial role in cancer progression. However little is known about the complex underlying molecular mechanisms that occur within the tumor microenvironment. Fibroblasts are the major stromal cells with multiple roles, especially towards both the extracellular matrix and the neighbouring cell population, including neoplastic cells. Consequently, proteomic analyses would provide a wider resource for a better understanding of the potential modulating effects exerted by fibroblasts on cancer cells. In this report we describe the effects of fibroblast stimulation on the breast cancer cell line (8701-BC) proteomics, using a trans-well co-culture system. Our results clearly indicate that fibroblasts induce considerable proteomic modulations on 8701-BC, mainly in the cytoskeleton proteins and glycolytic enzymes. Additionally, fibroblast-conditioned medium increased neoplastic cell proliferation and invasion with a concurrent up-regulation of the c-myc oncogene. Collectively these results suggest that fibroblast stimulation may enhance the malignant potential of breast cancer cells in vitro.
Settore BIO/06 - Anatomia Comparata E Citologia
Cancemi, P., Albanese, N., DI CARA, G., Marabeti, M., Costantini, F., Minafra, S., et al. (2010). Multiple changes induced by fibroblasts on breast cancer cells. CONNECTIVE TISSUE RESEARCH, 51 [10.3109/03008200903100651].
File in questo prodotto:
File Dimensione Formato  
10.Connec Tissue Res 2010.pdf

accesso aperto

Dimensione 835.81 kB
Formato Adobe PDF
835.81 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/35701
Citazioni
  • ???jsp.display-item.citation.pmc??? 11
  • Scopus 20
  • ???jsp.display-item.citation.isi??? 27
social impact