The impairment of humoral immune response in elderly humans has been extensively demonstrated. We have reported the increase of memory B cells (IgG+IgD−CD27−, double negative, DN) population in the elderly, in which there is also a typical inflammatory micro-environment. In order to evaluate whether this pro-inflammatory status could influence the trafficking phenotype of naïve/memory B cells, we have assessed the expression of CCR7, CCR6, CXCR3, CXCR4, CXCR5 and CD62L on naïve/memory B cell subpopulations in young and elderly subjects. Moreover, the combination of pro-inflammatory interleukin-21 (IL-21) and B cell receptor (BCR) stimulation enables B cells to produce and secrete granzyme B (GrB), which plays a critical role in early anti-viral immune responses, in the regulation of autoimmune mechanisms and in cancer immunosurveillance. Our data demonstrate that in the elderly, naïve/memory B cell populations present a different expression of the studied receptors that could be discussed in terms of “inflamm-aging”. In particular IgG+IgD−CD27− DN B cells show a tissue trafficking phenotype and they can be stimulated to produce GrB.

Bulati M, Buffa S, Martorana A, Candore G, Lio D, Caruso C, et al. (2014). Trafficking phenotype and production of granzyme B by double negative B cells (IgG(+)IgD(-)CD27(-)) in the elderly. EXPERIMENTAL GERONTOLOGY, 14, 123-129 [10.1016/j.exger.2013.12.011].

Trafficking phenotype and production of granzyme B by double negative B cells (IgG(+)IgD(-)CD27(-)) in the elderly.

BULATI, Matteo;BUFFA, Silvio;MARTORANA, Adriana;CANDORE, Giuseppina;LIO, Domenico;CARUSO, Calogero;COLONNA ROMANO, Giuseppina
2014

Abstract

The impairment of humoral immune response in elderly humans has been extensively demonstrated. We have reported the increase of memory B cells (IgG+IgD−CD27−, double negative, DN) population in the elderly, in which there is also a typical inflammatory micro-environment. In order to evaluate whether this pro-inflammatory status could influence the trafficking phenotype of naïve/memory B cells, we have assessed the expression of CCR7, CCR6, CXCR3, CXCR4, CXCR5 and CD62L on naïve/memory B cell subpopulations in young and elderly subjects. Moreover, the combination of pro-inflammatory interleukin-21 (IL-21) and B cell receptor (BCR) stimulation enables B cells to produce and secrete granzyme B (GrB), which plays a critical role in early anti-viral immune responses, in the regulation of autoimmune mechanisms and in cancer immunosurveillance. Our data demonstrate that in the elderly, naïve/memory B cell populations present a different expression of the studied receptors that could be discussed in terms of “inflamm-aging”. In particular IgG+IgD−CD27− DN B cells show a tissue trafficking phenotype and they can be stimulated to produce GrB.
Settore MED/04 - Patologia Generale
Settore MED/05 - Patologia Clinica
Bulati M, Buffa S, Martorana A, Candore G, Lio D, Caruso C, et al. (2014). Trafficking phenotype and production of granzyme B by double negative B cells (IgG(+)IgD(-)CD27(-)) in the elderly. EXPERIMENTAL GERONTOLOGY, 14, 123-129 [10.1016/j.exger.2013.12.011].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/99754
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