We investigated the antibacterial activity of Anemonia sulcata (Cnidaria, Anthozoa) tentacle and body acidic extracts. Biochemical purification consisted of first step on solid phase Sep-Pak C8 column followed by several HPLC runs on C18 column using different conditions. Anti-Micrococcus lysodeikticus activity has been detected in 40 % acetonitrile fractions. The resulting purified molecule from tentacles had a molecular mass determined by MALDI-TOF mass spectrum of 4946,299 Da and has been completely sequenced. Its aa sequence revealed identity with the Neurotoxin 2 (ATX-II), a Na+ channel blocking toxins. Consequently, ATX-II appeared to display a dual role as toxin and as antibacterial.
Trapani, M.R., Parisi, M.G., Toubiana, M., Coquet, L., Jouenne, T., Roch, P., et al. (2014). First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria). INVERTEBRATE SURVIVAL JOURNAL, 11.
First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria)
TRAPANI, Maria Rosa;PARISI, Maria Giovanna;CAMMARATA, Matteo
2014-01-01
Abstract
We investigated the antibacterial activity of Anemonia sulcata (Cnidaria, Anthozoa) tentacle and body acidic extracts. Biochemical purification consisted of first step on solid phase Sep-Pak C8 column followed by several HPLC runs on C18 column using different conditions. Anti-Micrococcus lysodeikticus activity has been detected in 40 % acetonitrile fractions. The resulting purified molecule from tentacles had a molecular mass determined by MALDI-TOF mass spectrum of 4946,299 Da and has been completely sequenced. Its aa sequence revealed identity with the Neurotoxin 2 (ATX-II), a Na+ channel blocking toxins. Consequently, ATX-II appeared to display a dual role as toxin and as antibacterial.
| File | Dimensione | Formato | |
|---|---|---|---|
|
ISJ335.pdf
accesso aperto
Dimensione
393.95 kB
Formato
Adobe PDF
|
393.95 kB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
