We investigated the antibacterial activity of Anemonia sulcata (Cnidaria, Anthozoa) tentacle and body acidic extracts. Biochemical purification consisted of first step on solid phase Sep-Pak C8 column followed by several HPLC runs on C18 column using different conditions. Anti-Micrococcus lysodeikticus activity has been detected in 40 % acetonitrile fractions. The resulting purified molecule from tentacles had a molecular mass determined by MALDI-TOF mass spectrum of 4946,299 Da and has been completely sequenced. Its aa sequence revealed identity with the Neurotoxin 2 (ATX-II), a Na+ channel blocking toxins. Consequently, ATX-II appeared to display a dual role as toxin and as antibacterial.
|Data di pubblicazione:||2014|
|Titolo:||First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria)|
|Autori:||Trapani, MR; Parisi, MG; Toubiana, M; Coquet, L; Jouenne, T; Roch, P; Cammarata, M|
|Tipologia:||Articolo su rivista|
|Citazione:||Trapani, M.R., Parisi, M.G., Toubiana, M., Coquet, L., Jouenne, T., Roch, P., et al. (2014). First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria). INVERTEBRATE SURVIVAL JOURNAL, 11.|
|Tipo:||Articolo in rivista|
|Appare nelle tipologie:||01 - Articolo su rivista|