Time-resolved wide-angle X-ray scattering (TR-WAXS) is a recently developed technique allowing to probe global structural changes of proteins in solution. We have used TR-WAXS to investigate large conformational changes of heme proteins (wild-type and mutant hemoglobin, neuroglobin, etc.) that cannot take place when these macromolecules are in a crystalline environment. Our data revealed detailed information on kinetic and thermodynamic properties of the investigated proteins and demonstrate the potentiality of the TR-WAXS technique.

Levantino, M., Cammarata, M., Vallone, B., Brunori, M., Cupane, A. (2013). Time-resolved X-ray scattering as a tool to probe heme proteins structural dynamics in solution. In 46th Course The Future of Dynamic Structural Science (pp.262-262).

Time-resolved X-ray scattering as a tool to probe heme proteins structural dynamics in solution

LEVANTINO, Matteo;CUPANE, Antonio
2013-01-01

Abstract

Time-resolved wide-angle X-ray scattering (TR-WAXS) is a recently developed technique allowing to probe global structural changes of proteins in solution. We have used TR-WAXS to investigate large conformational changes of heme proteins (wild-type and mutant hemoglobin, neuroglobin, etc.) that cannot take place when these macromolecules are in a crystalline environment. Our data revealed detailed information on kinetic and thermodynamic properties of the investigated proteins and demonstrate the potentiality of the TR-WAXS technique.
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
7-giu-2013
International School of Crystallography - 46th Course: The Future of Dynamic Structural Science
Erice, TP (Italy)
30/05/2013 - 08/06/2013
2013
1
Levantino, M., Cammarata, M., Vallone, B., Brunori, M., Cupane, A. (2013). Time-resolved X-ray scattering as a tool to probe heme proteins structural dynamics in solution. In 46th Course The Future of Dynamic Structural Science (pp.262-262).
Proceedings (atti dei congressi)
Levantino, M; Cammarata, M; Vallone, B; Brunori, M; Cupane, A.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/80304
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