Time-resolved wide-angle X-ray scattering (TR-WAXS) is a recently developed technique allowing to probe global structural changes of proteins in solution. We have used TR-WAXS to investigate large conformational changes of heme proteins (wild-type and mutant hemoglobin, neuroglobin, etc.) that cannot take place when these macromolecules are in a crystalline environment. Our data revealed detailed information on kinetic and thermodynamic properties of the investigated proteins and demonstrate the potentiality of the TR-WAXS technique.
Levantino, M., Cammarata, M., Vallone, B., Brunori, M., Cupane, A. (2013). Time-resolved X-ray scattering as a tool to probe heme proteins structural dynamics in solution. In 46th Course The Future of Dynamic Structural Science (pp.262-262).
Time-resolved X-ray scattering as a tool to probe heme proteins structural dynamics in solution
LEVANTINO, Matteo;CUPANE, Antonio
2013-01-01
Abstract
Time-resolved wide-angle X-ray scattering (TR-WAXS) is a recently developed technique allowing to probe global structural changes of proteins in solution. We have used TR-WAXS to investigate large conformational changes of heme proteins (wild-type and mutant hemoglobin, neuroglobin, etc.) that cannot take place when these macromolecules are in a crystalline environment. Our data revealed detailed information on kinetic and thermodynamic properties of the investigated proteins and demonstrate the potentiality of the TR-WAXS technique.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.