To study the pathogen-host relationship, we used the model of the entomopathogenic bacterium Bacillus thuringiensis (Bt) and Rhynchophorus ferrugineus, a quarantine pest that attacks palm trees. In particular, we focused on the Bt stress-induced infections. We studied the effect of Bt on larval and adult growth, and on the expression of the heat shock proteins (HSPs), rapidly synthesized in the cell after exposure to stress including pathogens. Bt has negative effects on larval and adult growth, on total hemocytes counts and on the hemocyte type. HSP70 was evaluated in the supernatant of the brain lysate obtained from larvae and adults fed with sublethal doses of Bt. HSP70 expression was modulated in time (3h, 6h, 12h, 24h) in response to Bt ingestion, highlighting that Bt is a stress factor for the R. ferrugineus. Further investigation is needed to understand the possible correlation between the reduction of hemocytes and HSP70 modulation. The potential costs of the different bioassay used will be compared.
Russo, D., Celi, M., Arizza, V., Vazzana, M., & Manachini, B. (2013). Bacillus thuringiensis treatment modulate the HSP70 expression in larva and adult brain of Rhunchophorus ferrugineus. In XIV Meeting of the Italian Association of Comparative and Developmental Immulology.
Autori: | Russo, D.; Celi, M.; Arizza, V.; Vazzana, M.; Manachini, B. |
Titolo: | Bacillus thuringiensis treatment modulate the HSP70 expression in larva and adult brain of Rhunchophorus ferrugineus |
Settore Scientifico Disciplinare: | Settore BIO/05 - Zoologia |
Data di creazione: | 2013-02-15 |
Nome del convegno: | XIV Meeting of the Italian Association of Comparative and Developmental Immulology |
Luogo del convegno: | Palermo |
Anno del convegno: | February 14 - 16, 2013 |
Data di pubblicazione: | 2013 |
Numero di pagine: | 1 |
Citazione: | Russo, D., Celi, M., Arizza, V., Vazzana, M., & Manachini, B. (2013). Bacillus thuringiensis treatment modulate the HSP70 expression in larva and adult brain of Rhunchophorus ferrugineus. In XIV Meeting of the Italian Association of Comparative and Developmental Immulology. |
Tipologia: | 0 - Proceedings (TIPOLOGIA NON ATTIVA) |
Appare nelle tipologie: | 0 - Proceedings (TIPOLOGIA NON ATTIVA) |
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