A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Staphylococcus aureus and enterococci to reduce the cost of using lysostaphin. This protocol reduces the expenses of PFGE typing of S. aureus and enterococci as it removes the use of lysostaphin during the spheroplast formation from these bacteria.

Mehdi Feizabadi, M., Ghodousi, A., Nomanpour, B., Omrani, M., & Shahcheraghi, F. (2011). Development of a modified DNA extraction method for pulsed-field gel electrophoresis analysis of Staphylococcus aureus and enterococci without using lysostaphin. JOURNAL OF MICROBIOLOGICAL METHODS, 84.

Development of a modified DNA extraction method for pulsed-field gel electrophoresis analysis of Staphylococcus aureus and enterococci without using lysostaphin

GHODOUSI, Arash;
2011

Abstract

A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Staphylococcus aureus and enterococci to reduce the cost of using lysostaphin. This protocol reduces the expenses of PFGE typing of S. aureus and enterococci as it removes the use of lysostaphin during the spheroplast formation from these bacteria.
Settore MED/07 - Microbiologia E Microbiologia Clinica
http://www.sciencedirect.com/science/article/pii/S0167701210003805
Mehdi Feizabadi, M., Ghodousi, A., Nomanpour, B., Omrani, M., & Shahcheraghi, F. (2011). Development of a modified DNA extraction method for pulsed-field gel electrophoresis analysis of Staphylococcus aureus and enterococci without using lysostaphin. JOURNAL OF MICROBIOLOGICAL METHODS, 84.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/77582
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