Double Component Deposition (DCD) technology to assist polymeric isotropic Mitral Valve fabrication

The tricuspid and mitral valves (MVs) stand out from the semilunar valves because of their unique geometrical and mechanical features, posing significant challenges for replicating native valve functionality through tissue engineered (TE) polymeric substitutes. Acellular TE approaches have emerged as a promising solution, particularly for pediatric patients, offering the potential for native tissue regeneration and long-term functionality. One of the critical barrier to the clinical adoption of atrioventricular polymeric valves lies in their inability to withstand structural and hemodynamic stresses. Achieving a balance between material robustness and biocompatibility, while ensuring appropriate leaflet mechanics is essential for leaflets durability and mobility. Through electrospinning (ES) we optimized the fabrication of an engineered MV using double-component deposition (DCD) technology, leveraging electrostatic simulation which allow the identification of the Double Component colletor with the more homogeneous surface electrical field (EF). By experimenting with collectors of varying plastic-to-metal ratios, we fine-tuned the thickness of the valve leaflets, charactering the overall fabrication process. The results highlight the potential in control the deposition outcome of the electrospun engineered MV.