Investigating protein structural changes inside the cell is a major goal in molecular biology. Here we show that time-resolved wide-angle X-ray scattering is a valuable tool for this purpose. Hemoglobin has been chosen as a model system and its tertiary and quaternary conformational changes following laser flash-photolysis have been tracked in intact red blood cells with nanosecond time resolution.
Spilotros, A., Levantino, M., Schirò, G., Cammarata, M., Wulff, M., Cupane, A. (2012). Probing in cell protein structural changes with time-resolved X-ray scattering. SOFT MATTER, 8(24), 6434-6437 [10.1039/C2SM25676B].
Probing in cell protein structural changes with time-resolved X-ray scattering
SPILOTROS, Alessandro;LEVANTINO, Matteo;SCHIRO', Giorgio;CUPANE, Antonio
2012-01-01
Abstract
Investigating protein structural changes inside the cell is a major goal in molecular biology. Here we show that time-resolved wide-angle X-ray scattering is a valuable tool for this purpose. Hemoglobin has been chosen as a model system and its tertiary and quaternary conformational changes following laser flash-photolysis have been tracked in intact red blood cells with nanosecond time resolution.
| File | Dimensione | Formato | |
|---|---|---|---|
|
SoftMatter_2012_8_2434_Spilotros_et_al.pdf
Open Access dal 29/07/2012
Descrizione: Articolo principale
Dimensione
435.43 kB
Formato
Adobe PDF
|
435.43 kB | Adobe PDF | Visualizza/Apri |
|
SoftMatter_2012_8_2434_SuppInfo.pdf
Open Access dal 29/07/2012
Descrizione: Informazioni di supporto
Dimensione
175.59 kB
Formato
Adobe PDF
|
175.59 kB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
