Investigating protein structural changes inside the cell is a major goal in molecular biology. Here we show that time-resolved wide-angle X-ray scattering is a valuable tool for this purpose. Hemoglobin has been chosen as a model system and its tertiary and quaternary conformational changes following laser flash-photolysis have been tracked in intact red blood cells with nanosecond time resolution.
Spilotros, A., Levantino, M., Schiro', G., Cammarata, M., Wulff, M., & Cupane, A. (2012). Probing in cell protein structural changes with time-resolved X-ray scattering. SOFT MATTER, 8(24), 6434-6437.
Data di pubblicazione: | 2012 |
Titolo: | Probing in cell protein structural changes with time-resolved X-ray scattering |
Autori: | |
Citazione: | Spilotros, A., Levantino, M., Schiro', G., Cammarata, M., Wulff, M., & Cupane, A. (2012). Probing in cell protein structural changes with time-resolved X-ray scattering. SOFT MATTER, 8(24), 6434-6437. |
Rivista: | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1039/C2SM25676B |
Abstract: | Investigating protein structural changes inside the cell is a major goal in molecular biology. Here we show that time-resolved wide-angle X-ray scattering is a valuable tool for this purpose. Hemoglobin has been chosen as a model system and its tertiary and quaternary conformational changes following laser flash-photolysis have been tracked in intact red blood cells with nanosecond time resolution. |
URL: | http://pubs.rsc.org/en/content/articlelanding/2012/sm/c2sm25676b |
Settore Scientifico Disciplinare: | Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin) |
Appare nelle tipologie: | 1.01 Articolo in rivista |
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