Investigating protein structural changes inside the cell is a major goal in molecular biology. Here we show that time-resolved wide-angle X-ray scattering is a valuable tool for this purpose. Hemoglobin has been chosen as a model system and its tertiary and quaternary conformational changes following laser flash-photolysis have been tracked in intact red blood cells with nanosecond time resolution.

Spilotros, A., Levantino, M., Schirò, G., Cammarata, M., Wulff, M., Cupane, A. (2012). Probing in cell protein structural changes with time-resolved X-ray scattering. SOFT MATTER, 8(24), 6434-6437 [10.1039/C2SM25676B].

Probing in cell protein structural changes with time-resolved X-ray scattering

SPILOTROS, Alessandro;LEVANTINO, Matteo;SCHIRO', Giorgio;CUPANE, Antonio
2012-01-01

Abstract

Investigating protein structural changes inside the cell is a major goal in molecular biology. Here we show that time-resolved wide-angle X-ray scattering is a valuable tool for this purpose. Hemoglobin has been chosen as a model system and its tertiary and quaternary conformational changes following laser flash-photolysis have been tracked in intact red blood cells with nanosecond time resolution.
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
http://pubs.rsc.org/en/content/articlelanding/2012/sm/c2sm25676b
Spilotros, A., Levantino, M., Schirò, G., Cammarata, M., Wulff, M., Cupane, A. (2012). Probing in cell protein structural changes with time-resolved X-ray scattering. SOFT MATTER, 8(24), 6434-6437 [10.1039/C2SM25676B].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/63231
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