Objective: To evaluate the hypothesis of human dental pulp cell death during orthodontic treatement (O.T) and the degree of apoptosis through the expression level of the proteins Caspase-3,-9, TUNEL and HSP60. Materials and methods: Human dental Pulp were coming from both male and female patients (N=20; age 10-14years). The technique used was the Straight Wire, which involves Nickel-Titanium or Steel archwires. The increase of pressure applied on teeth was gradual. Some patients were subjected to a premolar extraction after 3 months treatment, and others after 6 months. Samples were Bouin-fixed, paraffin-embedded and afterwards processed for immunohistochemistry using anti-caspase-3,-9 antibody, anti-HSP60 and TUNEL. Results: Increasing of caspase-3,-9 expression occurred in 3 and 6 months O.T. samples, while in control pulps positivity was detected mainly at odontoblasts level. HSP60 was not expressed in control samples and very weak in 3 months O.T. samples, it was expressed instead in 6 month O.T. samples, the expression of Tunel is evident in all samples but increased at 3 and 6 months specimens . Conclusion: Our hypothesis is supported by the increasing expression of Caspase-3,-9 , HSP60 and Tunel after 3 and 6 months of orthodontic traction revealing a time-dependent relationship.

LEONE, A., CAPPELLO, F., BONAVENTURA, G., BUSCEMI, M. (2011). Does the orthodontic treatment induces dental pulp cellular death? Caspase-3 and-9, HSP60 and TUNEL expression. In IJAE (pp.99-99). Firenza : Firenze University Press.

Does the orthodontic treatment induces dental pulp cellular death? Caspase-3 and-9, HSP60 and TUNEL expression

LEONE, Angelo;CAPPELLO, Francesco;BONAVENTURA, Giuseppe;BUSCEMI, Maria
2011-01-01

Abstract

Objective: To evaluate the hypothesis of human dental pulp cell death during orthodontic treatement (O.T) and the degree of apoptosis through the expression level of the proteins Caspase-3,-9, TUNEL and HSP60. Materials and methods: Human dental Pulp were coming from both male and female patients (N=20; age 10-14years). The technique used was the Straight Wire, which involves Nickel-Titanium or Steel archwires. The increase of pressure applied on teeth was gradual. Some patients were subjected to a premolar extraction after 3 months treatment, and others after 6 months. Samples were Bouin-fixed, paraffin-embedded and afterwards processed for immunohistochemistry using anti-caspase-3,-9 antibody, anti-HSP60 and TUNEL. Results: Increasing of caspase-3,-9 expression occurred in 3 and 6 months O.T. samples, while in control pulps positivity was detected mainly at odontoblasts level. HSP60 was not expressed in control samples and very weak in 3 months O.T. samples, it was expressed instead in 6 month O.T. samples, the expression of Tunel is evident in all samples but increased at 3 and 6 months specimens . Conclusion: Our hypothesis is supported by the increasing expression of Caspase-3,-9 , HSP60 and Tunel after 3 and 6 months of orthodontic traction revealing a time-dependent relationship.
28-set-2011
65° Congresso della Società Italiana di Anatomia e Istologia
Padova
27-29 settembre
65
1
LEONE, A., CAPPELLO, F., BONAVENTURA, G., BUSCEMI, M. (2011). Does the orthodontic treatment induces dental pulp cellular death? Caspase-3 and-9, HSP60 and TUNEL expression. In IJAE (pp.99-99). Firenza : Firenze University Press.
Proceedings (atti dei congressi)
LEONE, A; CAPPELLO, F; BONAVENTURA, G; BUSCEMI, M
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/60217
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact