The increasing industrial use of vanadium (V), as well as its recent medical use in various pathologies has intensified its environmental release, making it an emerging pollutant [1]. The sea urchin embryo has long been used to study the effects induced by metals, including V. In this study we used an integrated approach that correlates the biological effects on embryo development with proteolytic activities of gelatinases that could better reflect any metal induced imbalances. V-exposure caused morphological/morphometric aberrations, mainly concerning the correct dis-tribution of embryonic cells, the development of the skeleton and the embryo volume [2]. Moreover, V induced a concentration change in all the gelatinases expressed during embryo development and a reduction in their total proteolytic activity. The presence of three MMPs-like gelatinases (MMP-2, -9 and -14) was also demonstrated and their levels depended on V-concentration. In particular, the MMP-14-like protein modified its expression level during embryo development in a time and dose dependent manner. This enzyme also showed a specific localization on filopodia, suggesting that primary mesenchyme cells (PMCs) could be responsible for it synthesis. In conclusion, these results indicate that an integrated study among morphology/morphometry, proteolytic activity and MMP-14 expression constitutes an important response profile to V-action

Roberto Chiarelli, Chiara Martino, Rosaria Scudiero, Maria Carmela Roccheri, Fabiana Geraci (2023). Proteolytic activity and MMP-14-like protein levels are affected by Vanadium in Paracentrotus lividus Embryo. In Proteolytic activity and MMP-14-like protein levels are affected by Vanadium in Paracentrotus lividus Embryo (pp. 35-35). Palermo.

Proteolytic activity and MMP-14-like protein levels are affected by Vanadium in Paracentrotus lividus Embryo

Roberto Chiarelli
Primo
Membro del Collaboration Group
;
Chiara Martino
Secondo
Membro del Collaboration Group
;
Rosaria Scudiero
Membro del Collaboration Group
;
Maria Carmela Roccheri
Penultimo
Membro del Collaboration Group
;
Fabiana Geraci
Ultimo
Membro del Collaboration Group
2023-01-12

Abstract

The increasing industrial use of vanadium (V), as well as its recent medical use in various pathologies has intensified its environmental release, making it an emerging pollutant [1]. The sea urchin embryo has long been used to study the effects induced by metals, including V. In this study we used an integrated approach that correlates the biological effects on embryo development with proteolytic activities of gelatinases that could better reflect any metal induced imbalances. V-exposure caused morphological/morphometric aberrations, mainly concerning the correct dis-tribution of embryonic cells, the development of the skeleton and the embryo volume [2]. Moreover, V induced a concentration change in all the gelatinases expressed during embryo development and a reduction in their total proteolytic activity. The presence of three MMPs-like gelatinases (MMP-2, -9 and -14) was also demonstrated and their levels depended on V-concentration. In particular, the MMP-14-like protein modified its expression level during embryo development in a time and dose dependent manner. This enzyme also showed a specific localization on filopodia, suggesting that primary mesenchyme cells (PMCs) could be responsible for it synthesis. In conclusion, these results indicate that an integrated study among morphology/morphometry, proteolytic activity and MMP-14 expression constitutes an important response profile to V-action
MMP-14-like protein
Vanadium
Paracentrotus lividus embryo
978-88-942066-1-6
Roberto Chiarelli, Chiara Martino, Rosaria Scudiero, Maria Carmela Roccheri, Fabiana Geraci (2023). Proteolytic activity and MMP-14-like protein levels are affected by Vanadium in Paracentrotus lividus Embryo. In Proteolytic activity and MMP-14-like protein levels are affected by Vanadium in Paracentrotus lividus Embryo (pp. 35-35). Palermo.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/579228
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