The current experiments were undertaken to develop a method for regenerating doubled haploid (DH) plants of Eriobotrya japonica (Thunb.), Rosaceae, subfamily Maloideae through isolated microspore culture of nine loquat cultivars. Protocols were developed for isolation and culture of loquat microspores. Isolated microspores in vitro cultured started dividing and developing multicellular, globular and irregular structures. After 4 weeks of culture, the responding microspores produced yellowish/white callus. The embryogenic response of microspores was highly dependent on the cultivar and on the medium composition.
Padoan, D., Chiancone, B., Germana', M., Sha Valli Khan, P., Barany, I., Risueño, M., et al. (2011). First stages of microspore reprogramming to embryogenesis through isolated microspore culture in loquat.. In Proc. III International Symposium on Loquat. Acta Horticulturae 887 (pp. 285-290) [10.17660/ActaHortic.2011.887.49].
First stages of microspore reprogramming to embryogenesis through isolated microspore culture in loquat.
GERMANA', Maria;
2011-01-01
Abstract
The current experiments were undertaken to develop a method for regenerating doubled haploid (DH) plants of Eriobotrya japonica (Thunb.), Rosaceae, subfamily Maloideae through isolated microspore culture of nine loquat cultivars. Protocols were developed for isolation and culture of loquat microspores. Isolated microspores in vitro cultured started dividing and developing multicellular, globular and irregular structures. After 4 weeks of culture, the responding microspores produced yellowish/white callus. The embryogenic response of microspores was highly dependent on the cultivar and on the medium composition.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
