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EnglishIn this work we report the effects of exposure of mammalian cells to α-pinene, a bicyclic monoterpene founded in essential oils and used in insecticides, solvents, perfumes, etc.. Morphological analysis, performed in V79 cells exposed to increasing doses(25μM up to 50μM) of α-pinene, indicated a increase of dose-related nuclear abnormalities; apoptotic cells were seen at higher doses. Immunofluorescence with anti β- tubulin antibody showed that monoterpene induced genomic instability by interfering with mitotic process; in fact, 50% (vs 19% in the control cells) of irregular mitosis with multipolar or not correctly localized spindles were detected, suggesting that α-pinene affects cell stability by disturbing chromosome segregation. Cytogenetic analysis demonstrated that frequency of hypodiploid metaphases increased in a dose-dependent manner and, moreover, α-pinene induced endoreduplicated cells and double strand breaks. Alkaline comet confirmed that monoterpene exposure induced DNA lesions; in fact, OTM increased significantly in a dose-dependent manner. In order to assess whether the severe DNA damage evidenced by comet assay was originated through the ROS production, cells were incubated with CM-H2DCFDA and then analysed by flow cytometry. Results demonstrated an increase in fluorescence intensity after α-pinene treatment indicating an increased oxidative stress. On the whole, these findings strongly suggest that α-pinene is able to compromise genome stability preferentially through mitotic alterations and to damage DNA through reactive oxigen species production.
| Autori: | Barbata, G.; Caradonna, F.; Catanzaro, I.; Saverini, M.; Sciandrello, G. |
| Titolo: | Biological effects of alpha-pinene in cultured mammalian cells |
| Settore Scientifico Disciplinare: | Settore BIO/18 - Genetica |
| Data di creazione: | 2009-12-17 |
| Nome del convegno: | Excerpts from DBCS: VII congresso Nazionale del Dipartimento di Biologia Cellulare e dello Sviluppo |
| Luogo del convegno: | Palermo |
| Anno del convegno: | 17-18 Dicembre 2009 |
| Numero del convegno: | VII |
| Data di pubblicazione: | 2009 |
| Numero di pagine: | 1 |
| URL: | http://www.unipa.it/dipbio/congresso2009/congresso2009.html |
| Citazione: | Barbata, G., Caradonna, F., Catanzaro, I., Saverini, M., & Sciandrello, G. (2009). Biological effects of alpha-pinene in cultured mammalian cells. In Excerpts from DBCS (pp.33-33). Palermo. |
| Tipologia: | Proceedings (atti dei congressi) |
| Appare nelle tipologie: | 0 - Proceedings (TIPOLOGIA NON ATTIVA) |
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| Poster congresso DBCS 09.pdf | PDF del poster congresso DBCS 2009 Barbata et al | N/A | Administrator Richiedi una copia |
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