Objectives. To evaluate the antibacterial activity of four endodontic sealers: one epoxy resin sealer (AH Plus), two zinc oxide eugenol (ZOE)-based sealers (Endomethasone, Pulp Canal Sealer), and one sealer containing both ZOE and orthophenilphenol (Vcanalare). Methods. A direct contact test (DCT) was performed. A 10 ml suspension of Enterococcus faecalis was placed on the test material 20 min, 24 h and 7 days after mixing. Bacteria were allowed to directly contact the sealers for 1 h at 37 8C. Bacterial growth was then spectrophotometrically measured every 30 min for 7 h, and again after 24 h as well. Results. All freshly mixed sealers showed complete inhibition of bacterial growth. Similar results were obtained with the 24-h-old samples, with the exception of AH Plus. Vcanalare was the only sealer still inhibiting bacterial growth 7 days after mixing. Conclusions. The antimicrobial activity of the tested sealers depends on the time interval between mixing and testing. All sealers exhibit bactericidal effect when freshly mixed, but only Vcanalare extended this effect until 7 days after setting.

Pizzo, G., Giammanco, G.M., Cumbo, E., Nicolosi, G., Gallina, G. (2006). In vitro antibacterial activity of endodontic sealers. JOURNAL OF DENTISTRY, 34, 35-40 [10.1016/j.jdent.2005.03.001].

In vitro antibacterial activity of endodontic sealers

PIZZO, Giuseppe;GIAMMANCO, Giovanni;CUMBO, Enzo Maria Giuseppe;GALLINA, Giuseppe
2006-01-01

Abstract

Objectives. To evaluate the antibacterial activity of four endodontic sealers: one epoxy resin sealer (AH Plus), two zinc oxide eugenol (ZOE)-based sealers (Endomethasone, Pulp Canal Sealer), and one sealer containing both ZOE and orthophenilphenol (Vcanalare). Methods. A direct contact test (DCT) was performed. A 10 ml suspension of Enterococcus faecalis was placed on the test material 20 min, 24 h and 7 days after mixing. Bacteria were allowed to directly contact the sealers for 1 h at 37 8C. Bacterial growth was then spectrophotometrically measured every 30 min for 7 h, and again after 24 h as well. Results. All freshly mixed sealers showed complete inhibition of bacterial growth. Similar results were obtained with the 24-h-old samples, with the exception of AH Plus. Vcanalare was the only sealer still inhibiting bacterial growth 7 days after mixing. Conclusions. The antimicrobial activity of the tested sealers depends on the time interval between mixing and testing. All sealers exhibit bactericidal effect when freshly mixed, but only Vcanalare extended this effect until 7 days after setting.
2006
Settore MED/07 - Microbiologia E Microbiologia Clinica
Settore MED/28 - Malattie Odontostomatologiche
Pizzo, G., Giammanco, G.M., Cumbo, E., Nicolosi, G., Gallina, G. (2006). In vitro antibacterial activity of endodontic sealers. JOURNAL OF DENTISTRY, 34, 35-40 [10.1016/j.jdent.2005.03.001].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/44313
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