For the valorization of lignocellulosic biomass, a key step is unlocking the conversion of glucose to suitable chemical platforms. To this aim, we herein describe the sequential chemo-enzymatic conversion of glucose into 5-hydroxymethylfurfural (5-HMF), in two steps: glucose isomerization to fructose, catalyzed by glucose isomerase (GI) and fructose dehydration to 5-HMF promoted by Amberlyst 15. The novelty of our approach lies in the use of crude cell extracts of Streptomyces coelicolor showing GI activity, as isomerization catalysts, along with a commercial immobilized GI. Under optimized reaction conditions, we obtained a conversion to 5-HMF (C5-HMF) from glucose of 50% in the H2O/[bmim][Cl]0.5[BF4]0.5 1:3 w-w ternary mixture. We also carried out both reactions under sonochemical activation, obtaining a conversion to fructose (Cfructose) of 50% and C5-HMF 74% for the isomerization and dehydration respectively, with a drastic reduction in reaction time and temperature. In this case, the amount of substrate processed was 50 times higher than in silent conditions.

Marullo S., Sutera A., Gallo G., Billeci F., Rizzo C., & D'Anna F. (2020). Chemo-enzymatic Conversion of Glucose in 5-Hydroxymethylfurfural: The Joint Effect of Ionic Liquids and Ultrasound. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 8(30), 11204-11214 [10.1021/acssuschemeng.0c02555].

Chemo-enzymatic Conversion of Glucose in 5-Hydroxymethylfurfural: The Joint Effect of Ionic Liquids and Ultrasound

Marullo S.;Sutera A.;Gallo G.;Billeci F.;Rizzo C.;D'Anna F.
2020

Abstract

For the valorization of lignocellulosic biomass, a key step is unlocking the conversion of glucose to suitable chemical platforms. To this aim, we herein describe the sequential chemo-enzymatic conversion of glucose into 5-hydroxymethylfurfural (5-HMF), in two steps: glucose isomerization to fructose, catalyzed by glucose isomerase (GI) and fructose dehydration to 5-HMF promoted by Amberlyst 15. The novelty of our approach lies in the use of crude cell extracts of Streptomyces coelicolor showing GI activity, as isomerization catalysts, along with a commercial immobilized GI. Under optimized reaction conditions, we obtained a conversion to 5-HMF (C5-HMF) from glucose of 50% in the H2O/[bmim][Cl]0.5[BF4]0.5 1:3 w-w ternary mixture. We also carried out both reactions under sonochemical activation, obtaining a conversion to fructose (Cfructose) of 50% and C5-HMF 74% for the isomerization and dehydration respectively, with a drastic reduction in reaction time and temperature. In this case, the amount of substrate processed was 50 times higher than in silent conditions.
Settore CHIM/06 - Chimica Organica
Settore BIO/19 - Microbiologia Generale
Marullo S., Sutera A., Gallo G., Billeci F., Rizzo C., & D'Anna F. (2020). Chemo-enzymatic Conversion of Glucose in 5-Hydroxymethylfurfural: The Joint Effect of Ionic Liquids and Ultrasound. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 8(30), 11204-11214 [10.1021/acssuschemeng.0c02555].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/431420
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