Specific antibodies are essential tools for studying proteins as well as for diagnostic research in biomedicine. The egg yolk of immunized chicken is an inexpensive source of high-quality polyclonal antibodies. The 12-kDa Parietaria judaica 2 allergen was expressed as a fusion protein and was used to immunize Leghorn chickens. In this paper, we show, using 2-dimensional gel electrophoresis and immunoblotting, that chicken antibodies raised against a recombinant allergen can be used to recognize similar proteins from a pollen raw extract. Allergen identity was confirmed by nanoLC-nanospray-tandem mass spectrometry analysis. Our data demonstrate for the first time that a synergistic combination of molecular biology, 2-dimensional PAGE, and use of nonmammalian antibodies represents a powerful tool for reliable identification of allergens.
|Data di pubblicazione:||ago-2009|
|Titolo:||Production of an egg yolk antibody against Parietaria judaica 2 allergen.|
|Autori:||Alessandro, R; Gallo, A; Barranca, M; Principe, S; Taverna, S; Duro, G; Cassata, G; Becchi, M; Fontana, S; De Leo, G|
|Tipologia:||Articolo su rivista|
|Citazione:||Alessandro, R., Gallo, A., Barranca, M., Principe, S., Taverna, S., Duro, G., et al. (2009). Production of an egg yolk antibody against Parietaria judaica 2 allergen. POULTRY SCIENCE, 88(8), 1773-1778.|
|Tipo:||Articolo in rivista|
|Appare nelle tipologie:||01 - Articolo su rivista|