In the present study, we report the comparative proteome profiles of proteins solubilized from 37 breast cancer surgical tissues, normalized for the actin content. Blood-derived proteins were excluded from the analysis. Among the tumor-derived protein spots, a large proportion (39%) was found present in all patients. These included several glycolytic enzymes, detox and heat shock proteins, members of annexin and S100 protein families, cathepsin D, and two "rare" proteins, DDAH2 involved in the angiogenesis control, and the oncogene PARK7. Other proteins, such as psoriasin, galectin1, cofilin, peroredoxins, SH3L1, and others, showed sporadic presence and high expression level, which suggests their possible role for patient stratification.

Pucci, I., Cancemi, P., Albanese, N., DI CARA, G., Marabeti, M., Marrazzo, A., et al. (2008). New protein clustering of breast cancer tissue proteomics using actin content as a cellularity indicator. JOURNAL OF PROTEOME RESEARCH, 7(4), 1412-1418 [10.1021/pr700748m].

New protein clustering of breast cancer tissue proteomics using actin content as a cellularity indicator

CANCEMI, Patrizia;ALBANESE, Nadia Ninfa;DI CARA, Gianluca;MARRAZZO, Antonio;
2008

Abstract

In the present study, we report the comparative proteome profiles of proteins solubilized from 37 breast cancer surgical tissues, normalized for the actin content. Blood-derived proteins were excluded from the analysis. Among the tumor-derived protein spots, a large proportion (39%) was found present in all patients. These included several glycolytic enzymes, detox and heat shock proteins, members of annexin and S100 protein families, cathepsin D, and two "rare" proteins, DDAH2 involved in the angiogenesis control, and the oncogene PARK7. Other proteins, such as psoriasin, galectin1, cofilin, peroredoxins, SH3L1, and others, showed sporadic presence and high expression level, which suggests their possible role for patient stratification.
Settore BIO/06 - Anatomia Comparata E Citologia
Settore MED/18 - Chirurgia Generale
http://pubs.acs.org/doi/abs/10.1021/pr700748m
Pucci, I., Cancemi, P., Albanese, N., DI CARA, G., Marabeti, M., Marrazzo, A., et al. (2008). New protein clustering of breast cancer tissue proteomics using actin content as a cellularity indicator. JOURNAL OF PROTEOME RESEARCH, 7(4), 1412-1418 [10.1021/pr700748m].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/36400
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