Candida dubliniensis, an emerging oral pathogen, phenotypically resembles Candida albicans so closely that it is easily misidentified as such. The aim of the present study was to evaluate the usefulness of two phenotypic methods, growth at 45°C and 2,3,5-triphenyltetrazolium chloride (TTC) reduction, for confirming presumptive identification of C. dubliniensis and C. albicans by colony color on CHROMagar Candida (CAC) medium. A combination of these methods was used to establish the prevalence of oral C. dubliniensis in an Italian population of 45 human immunodeficiency virus (HIV)-infected subjects. Twenty-two samples (48.9%) were positive for yeasts on CAC medium producing a total of 37 fungal isolates. The colony color and 45°C growth ability test correctly identified all C. dubliniensis and C. albicans isolates (5/37, 13.5%, and 16/37, 43.2%, respectively), while assessment of TTC reduction misidentified one C. albicans isolate. The isolation rate of C. dubliniensis was 11.1% (5/45 patients). All of the C. dubliniensis isolates were highly susceptible to fluconazole (MIC = 0.5 μg/ml). The combination of CAC medium screening with growth at 45°C and TTC reduction tests may represent a simple, reliable and inexpensive identification protocol for C. dubliniensis.

Giammanco, G., Pizzo, G., Pecorella, S., Distefano, S., Pecoraro, V., Milici, M. (2002). Identification of Candida dubliniensis among oral yeast isolates from an italian population of human immunodeficiency virus-infected (HIV +) subjects. ORAL MICROBIOLOGY AND IMMUNOLOGY, 17(2), 89-94 [10.1046/j.0902-0055.2001.00097.x].

Identification of Candida dubliniensis among oral yeast isolates from an italian population of human immunodeficiency virus-infected (HIV +) subjects

Giammanco, Giovanni
;
Pizzo, G.;Pecorella, S.;Distefano, S.;Milici, M. E.
2002-01-01

Abstract

Candida dubliniensis, an emerging oral pathogen, phenotypically resembles Candida albicans so closely that it is easily misidentified as such. The aim of the present study was to evaluate the usefulness of two phenotypic methods, growth at 45°C and 2,3,5-triphenyltetrazolium chloride (TTC) reduction, for confirming presumptive identification of C. dubliniensis and C. albicans by colony color on CHROMagar Candida (CAC) medium. A combination of these methods was used to establish the prevalence of oral C. dubliniensis in an Italian population of 45 human immunodeficiency virus (HIV)-infected subjects. Twenty-two samples (48.9%) were positive for yeasts on CAC medium producing a total of 37 fungal isolates. The colony color and 45°C growth ability test correctly identified all C. dubliniensis and C. albicans isolates (5/37, 13.5%, and 16/37, 43.2%, respectively), while assessment of TTC reduction misidentified one C. albicans isolate. The isolation rate of C. dubliniensis was 11.1% (5/45 patients). All of the C. dubliniensis isolates were highly susceptible to fluconazole (MIC = 0.5 μg/ml). The combination of CAC medium screening with growth at 45°C and TTC reduction tests may represent a simple, reliable and inexpensive identification protocol for C. dubliniensis.
2002
Settore MED/07 - Microbiologia E Microbiologia Clinica
Settore MED/28 - Malattie Odontostomatologiche
Giammanco, G., Pizzo, G., Pecorella, S., Distefano, S., Pecoraro, V., Milici, M. (2002). Identification of Candida dubliniensis among oral yeast isolates from an italian population of human immunodeficiency virus-infected (HIV +) subjects. ORAL MICROBIOLOGY AND IMMUNOLOGY, 17(2), 89-94 [10.1046/j.0902-0055.2001.00097.x].
File in questo prodotto:
File Dimensione Formato  
gmgoralmicro.pdf

Solo gestori archvio

Descrizione: Articolo principale
Dimensione 118.51 kB
Formato Adobe PDF
118.51 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/344919
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 25
  • ???jsp.display-item.citation.isi??? 19
social impact