Tissue engineering is an emerging multidisciplinary field that aims at reproducing in vitro and/or in vivo tissues with morphological and functional features similar to the biological tissue of the human body. In this communication we report setting of three-dimensional structures able to mimic the extracellular matrix of the nervous system: we prepared Poly-L-Lactic Acid (PLLA) porous scaffolds via thermally induced phase separation (TIPS), and investigated the parameters that influence porosity, average pore size and degree of interconnection, i.e. polymer concentration, temperature and time of process. Astrocytes and brain capillary endothelial cells (BCECs) were cultured on these three-dimensional structures and tested for their ability to grow and survive on PLLA scaffolds. We analyzed in parallel the cell growth in 2D and 3D culture systems and observed the differences in cell morphology by fluorescence analysis: three-dimensional scaffolds have the ability to guide cell growth, provide support, encourage cell adhesion and proliferation. Astrocytes and BCECs adapted well to these porous matrices, not only remaining on the surface, but also penetrating inside the scaffolds. This 3D cell culture system could be further enriched to host two or three different brain cell types, in order to set an in vitro model of blood brain barrier, that may be useful for drug delivery studies, and for the formulation of new therapeutic strategies, to be used for the treatment of neurological diseases.
Zummo, F., Schiera, G., Carfì Pavia, F., Di Liegro, C., Fricano, A., Di Bella, M., et al. (2016). 3D cultures of rat astrocytes and brain capillary endothelial cells on Poly-L-lactic acid scaffolds. In LIBRO DEGLI ABSTRACT (pp.20-20).
Autori: | Zummo, F.; Schiera, G.; Carfì Pavia, F.; Di Liegro, C.; Fricano, A.; Di Bella, M.; Di Liegro, I. |
Titolo: | 3D cultures of rat astrocytes and brain capillary endothelial cells on Poly-L-lactic acid scaffolds |
Settore Scientifico Disciplinare: | Settore BIO/10 - Biochimica Settore BIO/06 - Anatomia Comparata E Citologia Settore BIO/13 - Biologia Applicata Settore ING-IND/22 - Scienza E Tecnologia Dei Materiali |
Nome del convegno: | IV Meeting di Biotecnologie, ricerca di base Interdisciplinare traslazionale in ambito Biomedico |
Luogo del convegno: | Palermo |
Anno del convegno: | dal 15-12-2016 al 16-12-2016 |
Numero del convegno: | IV |
Data di pubblicazione: | 2016 |
Numero di pagine: | 1 |
Citazione: | Zummo, F., Schiera, G., Carfì Pavia, F., Di Liegro, C., Fricano, A., Di Bella, M., et al. (2016). 3D cultures of rat astrocytes and brain capillary endothelial cells on Poly-L-lactic acid scaffolds. In LIBRO DEGLI ABSTRACT (pp.20-20). |
Tipologia: | 0 - Proceedings (TIPOLOGIA NON ATTIVA) |
Appare nelle tipologie: | 0 - Proceedings (TIPOLOGIA NON ATTIVA) |
File in questo prodotto:
File | Descrizione | Tipologia | Licenza | |
---|---|---|---|---|
libro abstractCNR 2016.pdf | N/A | Administrator Richiedi una copia |