INTRODUCTION: Post-transcriptional regulation of gene expression relies on RNA-binding proteins (RBPs), which regulate intracellular transport, stability, and translation of mRNAs . We previously identified a set of proteins which interact with mRNAs encoding H1° and H3.3 histones [2-5]. All these proteins are probably part of a ribonucleoprotein particle . Here we report more details on the expression and intracellular localization of some of these RBPs, during rat brain development and in isolated rat astrocytes. METHODS: Affinity chromatography was performed as already described . Preparation of total lysates and cellular sub-fractions was done as reported in . Possible co-localization of Hsc70 with CSD-C2 in cultured astrocytes was analysed by immunofluorescence microscopy. RESULTS: The presence of Hsc70 chaperone in the already identified ribonucleoprotein complex  was confirmed by affinity chromatography. We also found that the complex itself is present not only in the nuclear extracts, but also in the cytoplasmic fraction. Moreover, A1 and K hnRNPs, previously found in the complexes, were found to be differentially expressed and localized during rat brain maturation; an increase in A1 expression was also demonstrated in cultured astrocytes grown on a fibronectin-containing substrate. We finally report that sumoylated PIPPin, already found in neurons, is also present in the nuclei of cultured astrocytes. CONCLUSIONS: We confirmed the existence of a group of proteins able to interact with H1°/H3.3 mRNAs. These proteins are, however, differentially expressed during brain maturation and also show different subcellular localization.  Di Liegro et al. 2014, Int J Mol Med 33:747-62. ;  Scaturro et al. 1998, J Biol Chem 273:22788-91.;  Nastasi et al. 1999, J Biol Chem 274:24087-93.  Sala et al. 2007, Int J Mol Med 19:501-9.  Saladino et al. 2012, Int J Mol Med 29:141-5  Di Liegro et al. 2013, Neuroscience 229:71-6.
Di Liegro, C., Di Gregorio, A., Schiera, G., & Di Liegro, I. (2015). Expression and intracellular localization of H1° mRNA-containing complexes in developing rat brain and astrocytes. In ABSTRACT BOOK (pp.201-201).
|Autori:||Di Liegro, C.; Di Gregorio, A.; Schiera, G.; Di Liegro, I.|
|Titolo:||Expression and intracellular localization of H1° mRNA-containing complexes in developing rat brain and astrocytes|
|Settore Scientifico Disciplinare:||Settore BIO/06 - Anatomia Comparata E Citologia|
Settore BIO/10 - Biochimica
|Nome del convegno:||58th National Meeting of the Italian Society of Biochemistry and Molecular Biology|
|Luogo del convegno:||Urbino|
|Anno del convegno:||dal 14 settembre 2015 al 16 settembre 2015|
|Numero del convegno:||58|
|Data di pubblicazione:||2015|
|Numero di pagine:||1|
|Citazione:||Di Liegro, C., Di Gregorio, A., Schiera, G., & Di Liegro, I. (2015). Expression and intracellular localization of H1° mRNA-containing complexes in developing rat brain and astrocytes. In ABSTRACT BOOK (pp.201-201).|
|Tipologia:||0 - Proceedings (TIPOLOGIA NON ATTIVA)|
|Appare nelle tipologie:||0 - Proceedings (TIPOLOGIA NON ATTIVA)|