In plant mitochondria an essential mechanism for gene expression is RNA editing, often influencing the synthesis of functional proteins. RNA editing alters the linearity of genetic information transfer. Indeed it causes differences between RNAs and their coding DNA sequences that hinder both experimental and computational research of genes. Therefore common software tools for gene search, successfully applied to find canonical genes, often fail in discovering genes encrypted in the genome of plants. Here we propose a novel strategy useful to identify candidate coding sequences resulting from possible editing substitutions. In particular, we consider c!u substitutions leading to the creation of new start and stop codons in the mitochondrial DNA of a given input organism. We try to mimic the natural RNA editing mechanism, in order to generate candidate Open Reading Frame sequences that could code for novel, uncharacterized proteins. Results obtained analyzing the mtDNA of Oryza sativa a re supportive of this approach, since we identified thirteen Open Reading Frame sequences transcribed in Oryza, that do not correspond to already known proteins. Five of the corresponding amino acid sequences present high homologies with proteins already discovered in other organisms, whereas, for the remaining ones, no such homology was detected.

Fassetti, F., Giallombardo, C., Leone, O., Palopoli, L., Rombo, S.E., Saiardi, A. (2016). Discovering new proteins in plant mitochondria by RNA editing simulation. In Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOINFORMATICS 2016), (pp. 182-189). SCITEPRESS - Science and Technology Publications, Lda [10.5220/0005664901820189].

Discovering new proteins in plant mitochondria by RNA editing simulation

Giallombardo, Claudia;Rombo, SE;
2016-01-01

Abstract

In plant mitochondria an essential mechanism for gene expression is RNA editing, often influencing the synthesis of functional proteins. RNA editing alters the linearity of genetic information transfer. Indeed it causes differences between RNAs and their coding DNA sequences that hinder both experimental and computational research of genes. Therefore common software tools for gene search, successfully applied to find canonical genes, often fail in discovering genes encrypted in the genome of plants. Here we propose a novel strategy useful to identify candidate coding sequences resulting from possible editing substitutions. In particular, we consider c!u substitutions leading to the creation of new start and stop codons in the mitochondrial DNA of a given input organism. We try to mimic the natural RNA editing mechanism, in order to generate candidate Open Reading Frame sequences that could code for novel, uncharacterized proteins. Results obtained analyzing the mtDNA of Oryza sativa a re supportive of this approach, since we identified thirteen Open Reading Frame sequences transcribed in Oryza, that do not correspond to already known proteins. Five of the corresponding amino acid sequences present high homologies with proteins already discovered in other organisms, whereas, for the remaining ones, no such homology was detected.
978-989-758-170-0
Fassetti, F., Giallombardo, C., Leone, O., Palopoli, L., Rombo, S.E., Saiardi, A. (2016). Discovering new proteins in plant mitochondria by RNA editing simulation. In Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOINFORMATICS 2016), (pp. 182-189). SCITEPRESS - Science and Technology Publications, Lda [10.5220/0005664901820189].
File in questo prodotto:
File Dimensione Formato  
BIOINFORMATICS_2016_21.pdf

Solo gestori archvio

Dimensione 292.57 kB
Formato Adobe PDF
292.57 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/242805
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? ND
social impact