A dual drug-loaded HNT-CD glycocluster delivery system based on halloysite nanotubes and carbohydrate functionalized cyclodextrin was developed by a green protocol using solvent-free microwave irradiation. The nanohybrid was employed for concurrent load and release of silibinin and curcumin. The new delivery system was characterized by means of TGA, FT-IR spectroscopy, SEM and DLS. These techniques confirm the successful loading of the two drugs in the system. SEM and DLS measurements highlighted that the nanomaterial preserves a tubular structure with an average hydrodynamic radius of ca. 200 nm. The release of the drugs from the HNT glycocluster was investigated by means of UV-vis spectroscopy at two different pH values simulanting the typical physiological conditions of either gastric or intestinal fluids. Enzyme-linked lectin assays (ELLA) demonstrated that highly mannoside-cyclodextrins HNT entities display high affinity towards mannose selective ConA lectin. Biological assays showed that the new drug delivery system exhibits anti-proliferative activity against the investigated cell lines. Fluorescence microscopy confirmed ELLA results and it showed a high propensity of this drug delivery system to cross cell membranes and to penetrate into the cell nucleus. The results revealed that the synthesized multicavity system is a material of suitable size and nanoarchitecture to transport drugs into living cells.

Massaro, M., Riela, S., Baiamonte, C., Blanco, J., Giordano, C., Lo Meo, P., et al. (2016). Dual drug-loaded halloysite hybrid-based glycocluster for sustained release of hydrophobic molecules. RSC ADVANCES, 6(91), 87935-87944 [10.1039/c6ra14657k].

Dual drug-loaded halloysite hybrid-based glycocluster for sustained release of hydrophobic molecules

MASSARO, Marina;RIELA, Serena
;
BAIAMONTE, Concetta;GIORDANO, Carla;LO MEO, Paolo Maria Giuseppe;MILIOTO, Stefana;NOTO, Renato;PARISI, Filippo;PIZZOLANTI, Giuseppe;LAZZARA, Giuseppe
2016

Abstract

A dual drug-loaded HNT-CD glycocluster delivery system based on halloysite nanotubes and carbohydrate functionalized cyclodextrin was developed by a green protocol using solvent-free microwave irradiation. The nanohybrid was employed for concurrent load and release of silibinin and curcumin. The new delivery system was characterized by means of TGA, FT-IR spectroscopy, SEM and DLS. These techniques confirm the successful loading of the two drugs in the system. SEM and DLS measurements highlighted that the nanomaterial preserves a tubular structure with an average hydrodynamic radius of ca. 200 nm. The release of the drugs from the HNT glycocluster was investigated by means of UV-vis spectroscopy at two different pH values simulanting the typical physiological conditions of either gastric or intestinal fluids. Enzyme-linked lectin assays (ELLA) demonstrated that highly mannoside-cyclodextrins HNT entities display high affinity towards mannose selective ConA lectin. Biological assays showed that the new drug delivery system exhibits anti-proliferative activity against the investigated cell lines. Fluorescence microscopy confirmed ELLA results and it showed a high propensity of this drug delivery system to cross cell membranes and to penetrate into the cell nucleus. The results revealed that the synthesized multicavity system is a material of suitable size and nanoarchitecture to transport drugs into living cells.
Settore CHIM/06 - Chimica Organica
Settore CHIM/02 - Chimica Fisica
Settore MED/13 - Endocrinologia
Massaro, M., Riela, S., Baiamonte, C., Blanco, J., Giordano, C., Lo Meo, P., et al. (2016). Dual drug-loaded halloysite hybrid-based glycocluster for sustained release of hydrophobic molecules. RSC ADVANCES, 6(91), 87935-87944 [10.1039/c6ra14657k].
File in questo prodotto:
File Dimensione Formato  
RSC Adv., 2016, 6, 87935–87944.pdf

Solo gestori archvio

Descrizione: Articolo principale
Tipologia: Versione Editoriale
Dimensione 1.2 MB
Formato Adobe PDF
1.2 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/204537
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 50
  • ???jsp.display-item.citation.isi??? 48
social impact