ckground information: Bone marrow-derived mesenchymal stem cells (MSCs) reduce acute lung injury in animals challenged by bleomycin or bacterial lipopolysaccaride. It is not known, however, whether MSCs protect from ventilator- induced lung injury (VILI). Question of the study: Whether MSCs have a potential role in preventing or modulating VILI in healthy rats subjected to high-volume ventilation. Materials and methods: 24 Sprague-Dawley rats (250-300 g) were subjected to high- volume mechanical ventilation (25 ml/kg). MSCs (5x106) were intravenously or intratracheally administered (N=8 each) 30 min before starting over-ventilation and 8 rats were MSC-untreated. Spontaneously breathing anesthetised rats (N=8) served as controls. After 3 h of over-ventilation/control the animals were sacrificed and lung tissue and bronchoalveolar lavage fluid (BALF) were sampled for further analysis. Results: When compared with controls, MSC-untreated over-ventilated rats exhibited typical VILI features. Lung edema histological lung injury index, concentrations of total protein, interleukin-1β, macrophage inflammatory protein-2 and number of neutrophils in BALF and vascular cell adhesion protein-1 in lung tissue significantly increased in overventilated rats. All these indices of VILI moved significantly towards normalization in the rats treated with MSCs, whether intravenously or intratracheally. Answer to question: Both local and systemic pre-treatment with MSCs reduced VILI in a rat model.
|Data di pubblicazione:||2012|
|Titolo:||Pre-treatment with mesenchymal stem cells reduces ventilator-induced lung injury.|
|Citazione:||Chimenti, L., Luque, T., Bonsignore, M., Ramirez, J., Navajas, D., & Farré, R. (2012). Pre-treatment with mesenchymal stem cells reduces ventilator-induced lung injury. EUROPEAN RESPIRATORY JOURNAL, 40(4), 939-948.|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1183/09031936.00153211|
|Settore Scientifico Disciplinare:||Settore MED/10 - Malattie Dell'Apparato Respiratorio|
|Appare nelle tipologie:||1.01 Articolo in rivista|