ckground information: Bone marrow-derived mesenchymal stem cells (MSCs) reduce acute lung injury in animals challenged by bleomycin or bacterial lipopolysaccaride. It is not known, however, whether MSCs protect from ventilator- induced lung injury (VILI). Question of the study: Whether MSCs have a potential role in preventing or modulating VILI in healthy rats subjected to high-volume ventilation. Materials and methods: 24 Sprague-Dawley rats (250-300 g) were subjected to high- volume mechanical ventilation (25 ml/kg). MSCs (5x106) were intravenously or intratracheally administered (N=8 each) 30 min before starting over-ventilation and 8 rats were MSC-untreated. Spontaneously breathing anesthetised rats (N=8) served as controls. After 3 h of over-ventilation/control the animals were sacrificed and lung tissue and bronchoalveolar lavage fluid (BALF) were sampled for further analysis. Results: When compared with controls, MSC-untreated over-ventilated rats exhibited typical VILI features. Lung edema histological lung injury index, concentrations of total protein, interleukin-1β, macrophage inflammatory protein-2 and number of neutrophils in BALF and vascular cell adhesion protein-1 in lung tissue significantly increased in overventilated rats. All these indices of VILI moved significantly towards normalization in the rats treated with MSCs, whether intravenously or intratracheally. Answer to question: Both local and systemic pre-treatment with MSCs reduced VILI in a rat model.

Chimenti, L., Luque, T., Bonsignore, M.R., Ramirez, J., Navajas, D., & Farré, R. (2012). Pre-treatment with mesenchymal stem cells reduces ventilator-induced lung injury. EUROPEAN RESPIRATORY JOURNAL, 40(4), 939-948 [10.1183/09031936.00153211].

Pre-treatment with mesenchymal stem cells reduces ventilator-induced lung injury.

BONSIGNORE, Maria Rosaria;
2012

Abstract

ckground information: Bone marrow-derived mesenchymal stem cells (MSCs) reduce acute lung injury in animals challenged by bleomycin or bacterial lipopolysaccaride. It is not known, however, whether MSCs protect from ventilator- induced lung injury (VILI). Question of the study: Whether MSCs have a potential role in preventing or modulating VILI in healthy rats subjected to high-volume ventilation. Materials and methods: 24 Sprague-Dawley rats (250-300 g) were subjected to high- volume mechanical ventilation (25 ml/kg). MSCs (5x106) were intravenously or intratracheally administered (N=8 each) 30 min before starting over-ventilation and 8 rats were MSC-untreated. Spontaneously breathing anesthetised rats (N=8) served as controls. After 3 h of over-ventilation/control the animals were sacrificed and lung tissue and bronchoalveolar lavage fluid (BALF) were sampled for further analysis. Results: When compared with controls, MSC-untreated over-ventilated rats exhibited typical VILI features. Lung edema histological lung injury index, concentrations of total protein, interleukin-1β, macrophage inflammatory protein-2 and number of neutrophils in BALF and vascular cell adhesion protein-1 in lung tissue significantly increased in overventilated rats. All these indices of VILI moved significantly towards normalization in the rats treated with MSCs, whether intravenously or intratracheally. Answer to question: Both local and systemic pre-treatment with MSCs reduced VILI in a rat model.
Settore MED/10 - Malattie Dell'Apparato Respiratorio
Chimenti, L., Luque, T., Bonsignore, M.R., Ramirez, J., Navajas, D., & Farré, R. (2012). Pre-treatment with mesenchymal stem cells reduces ventilator-induced lung injury. EUROPEAN RESPIRATORY JOURNAL, 40(4), 939-948 [10.1183/09031936.00153211].
File in questo prodotto:
File Dimensione Formato  
ChimentiMSC2012.pdf

Solo gestori archvio

Dimensione 565.8 kB
Formato Adobe PDF
565.8 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/99297
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 38
  • ???jsp.display-item.citation.isi??? 33
social impact