Intra-tumor injection of immunotherapeutic agents is often the most effective, likely because of concomitant modification of tumor microenvironment. We tested an immunotherapeutic regimen consisting of CpG oligonucleotides and of adenovirus-mediated gene delivery of CCL16 chemokine directly into orthotopically implanted prostate tumors by ultrasound-guided injection, followed by systemic administration of an anti-IL-10R antibody. This combination treatment induced rapid stromal rearrangement, characterized by massive leukocyte infiltration and large areas of necrosis, a scenario that eventually led to complete tumor rejection and systemic immunity in 75 % of the treated mice. In vivo T lymphocyte depletion experiments demonstrated that the efficacy of CCL16/CpG/anti-IL-10R combination treatment relies upon CD8 T lymphocytes whereas CD4 T cells are dispensable. The results underlie the feasibility of echo-guided local immunotherapy of tumors located in visceral organs that are not easily accessible

Mauri, G., Chiodoni, C., Parenza, M., Arioli, I., Tripodo, C., Colombo, M. (2013). Ultrasound-guided intra-tumor injection of combined immunotherapy cures mice from orthotopic prostate cancer. CANCER IMMUNOLOGY, IMMUNOTHERAPY, 12(62), 1811-1819 [10.1007/s00262-013-1486-7].

Ultrasound-guided intra-tumor injection of combined immunotherapy cures mice from orthotopic prostate cancer.

TRIPODO, Claudio;
2013-01-01

Abstract

Intra-tumor injection of immunotherapeutic agents is often the most effective, likely because of concomitant modification of tumor microenvironment. We tested an immunotherapeutic regimen consisting of CpG oligonucleotides and of adenovirus-mediated gene delivery of CCL16 chemokine directly into orthotopically implanted prostate tumors by ultrasound-guided injection, followed by systemic administration of an anti-IL-10R antibody. This combination treatment induced rapid stromal rearrangement, characterized by massive leukocyte infiltration and large areas of necrosis, a scenario that eventually led to complete tumor rejection and systemic immunity in 75 % of the treated mice. In vivo T lymphocyte depletion experiments demonstrated that the efficacy of CCL16/CpG/anti-IL-10R combination treatment relies upon CD8 T lymphocytes whereas CD4 T cells are dispensable. The results underlie the feasibility of echo-guided local immunotherapy of tumors located in visceral organs that are not easily accessible
2013
Mauri, G., Chiodoni, C., Parenza, M., Arioli, I., Tripodo, C., Colombo, M. (2013). Ultrasound-guided intra-tumor injection of combined immunotherapy cures mice from orthotopic prostate cancer. CANCER IMMUNOLOGY, IMMUNOTHERAPY, 12(62), 1811-1819 [10.1007/s00262-013-1486-7].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/99029
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