Here we describe carbapenem resistance determinants in two Klebsiella pneumoniae isolates recovered from two hospitalised patients in the same intensive care unit of a cancer hospital in Cairo, Egypt. PCR and sequencing were used to detect and characterise β-lactamase genes. Clonal relationships between the isolates were analysed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The first K. pneumoniae isolate carried the blaNDM-1 gene and the second isolate carried the blaOXA-163 gene. Both isolates co-expressed the extended-spectrum β-lactamase CTX-M-15. The two isolates belonged to different sequence types (STs), ST11 and ST16, respectively. No history of travel was established for the two patients. The first identification of NDM-1-producing K. pneumoniae in Egypt adds further evidence to the spread of NDM-1-producing Gram-negative micro-organisms in North Africa. The additional detection of blaOXA-163 in a K. pneumoniae isolate confirms its endemic presence in a critical healthcare setting of this geographic area.

Abdelaziz, M., Bonura, C., Aleo, A., Fasciana, T., Mammina C (2013). NDM-1 and OXA-163 producing Klebsiella pneumoniae isolates in Cairo, Egypt, 2012. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 1(4), 213-215 [10.1016/j.jgar.2013.06.003].

NDM-1 and OXA-163 producing Klebsiella pneumoniae isolates in Cairo, Egypt, 2012

BONURA, Celestino;ALEO, Aurora;FASCIANA, Teresa Maria Assunta;MAMMINA, Caterina
2013-01-01

Abstract

Here we describe carbapenem resistance determinants in two Klebsiella pneumoniae isolates recovered from two hospitalised patients in the same intensive care unit of a cancer hospital in Cairo, Egypt. PCR and sequencing were used to detect and characterise β-lactamase genes. Clonal relationships between the isolates were analysed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The first K. pneumoniae isolate carried the blaNDM-1 gene and the second isolate carried the blaOXA-163 gene. Both isolates co-expressed the extended-spectrum β-lactamase CTX-M-15. The two isolates belonged to different sequence types (STs), ST11 and ST16, respectively. No history of travel was established for the two patients. The first identification of NDM-1-producing K. pneumoniae in Egypt adds further evidence to the spread of NDM-1-producing Gram-negative micro-organisms in North Africa. The additional detection of blaOXA-163 in a K. pneumoniae isolate confirms its endemic presence in a critical healthcare setting of this geographic area.
Settore MED/07 - Microbiologia E Microbiologia Clinica
Abdelaziz, M., Bonura, C., Aleo, A., Fasciana, T., Mammina C (2013). NDM-1 and OXA-163 producing Klebsiella pneumoniae isolates in Cairo, Egypt, 2012. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 1(4), 213-215 [10.1016/j.jgar.2013.06.003].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/98956
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