The PGMY-PCR for human papillomavirus (HPV) was evaluated, in parallel with nested PCR (nPCR), in samples with noted Hybrid Capture II (HCII) and MY-PCR results. PGMY-PCR detected HPV DNA in 2.5% of HCII-negative-MY-PCR-negative samples and in 71.7% of HCII-positive-MY-PCR-negative samples; also, it detected the MY-PCR-negative-nPCR-negative types HPV-42, HPV-44, HPV-51, HPV-87, and HPV-89.
GIOVANNELLI, L., A LAMA, CAPRA, G., GIORDANO, V., ARICO, P., AMMATUNA, P. (2004). Detection of Human Papillomavirus DNA in cervical samples: analysis of the new PGMY-PCR compared to Hybrid Capture II, MY-PCR and a Two-step nested PCR assay. JOURNAL OF CLINICAL MICROBIOLOGY, 42(8), 3861-3864 [10.1128/JCM.42.8.3861-3864.2004.].
Detection of Human Papillomavirus DNA in cervical samples: analysis of the new PGMY-PCR compared to Hybrid Capture II, MY-PCR and a Two-step nested PCR assay.
CAPRA, Giuseppina;AMMATUNA, Pietro
2004-01-01
Abstract
The PGMY-PCR for human papillomavirus (HPV) was evaluated, in parallel with nested PCR (nPCR), in samples with noted Hybrid Capture II (HCII) and MY-PCR results. PGMY-PCR detected HPV DNA in 2.5% of HCII-negative-MY-PCR-negative samples and in 71.7% of HCII-positive-MY-PCR-negative samples; also, it detected the MY-PCR-negative-nPCR-negative types HPV-42, HPV-44, HPV-51, HPV-87, and HPV-89.
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