Objective: To evaluate the prevalence of human papillomavirus (HPV) sperm infection and its correlation with sperm parameters in patients who attended a fertility clinic. Design: Cross-sectional clinical study. Setting: University-affiliated Reproductive Medicine Clinic. Patients: A total of 308 male partners of couples undergoing in vitro fertilization techniques. Interventions: Specimens of semen were collected from all patients. Main Outcome Measures: Sperm parameters were evaluated according to the World Health Organization manual. The presence of HPV-DNA was researched by the combined use of two HPV assays and a highly sensitive nested PCR assay, followed by HPV genotyping. To examine whether HPV was associated with the sperm, in situ hybridization (ISH) analysis was performed. Results: Results of HPV investigation were compared to sperm parameters and ISH analysis. Twenty-four out of 308 (7.8%) semen samples were HPV DNA positive but HPV infection does not seem to affect semen quality. Moreover, ISH revealed a clear HPV localization at the equatorial region of sperm head in infected samples. Conclusions: Oncogenic HPV genotypes were detected on spermatozoa from asymptomatic subjects but a role of the infection in male infertility was not demonstrated.

Schillaci, R., Capra, G., Bellavia, C., Ruvolo, G., Scazzone, C., Venezia, R., et al. (2013). Detection of oncogenic human papillomavirus genotypes on spermatozoa from male partners of infertile couples. FERTILITY AND STERILITY, 100, 1236-1240.

Detection of oncogenic human papillomavirus genotypes on spermatozoa from male partners of infertile couples

SCHILLACI, Rosaria;CAPRA, Giuseppina;SCAZZONE, Concetta;VENEZIA, Renato;PERINO, Antonino
2013

Abstract

Objective: To evaluate the prevalence of human papillomavirus (HPV) sperm infection and its correlation with sperm parameters in patients who attended a fertility clinic. Design: Cross-sectional clinical study. Setting: University-affiliated Reproductive Medicine Clinic. Patients: A total of 308 male partners of couples undergoing in vitro fertilization techniques. Interventions: Specimens of semen were collected from all patients. Main Outcome Measures: Sperm parameters were evaluated according to the World Health Organization manual. The presence of HPV-DNA was researched by the combined use of two HPV assays and a highly sensitive nested PCR assay, followed by HPV genotyping. To examine whether HPV was associated with the sperm, in situ hybridization (ISH) analysis was performed. Results: Results of HPV investigation were compared to sperm parameters and ISH analysis. Twenty-four out of 308 (7.8%) semen samples were HPV DNA positive but HPV infection does not seem to affect semen quality. Moreover, ISH revealed a clear HPV localization at the equatorial region of sperm head in infected samples. Conclusions: Oncogenic HPV genotypes were detected on spermatozoa from asymptomatic subjects but a role of the infection in male infertility was not demonstrated.
Settore MED/40 - Ginecologia E Ostetricia
Schillaci, R., Capra, G., Bellavia, C., Ruvolo, G., Scazzone, C., Venezia, R., et al. (2013). Detection of oncogenic human papillomavirus genotypes on spermatozoa from male partners of infertile couples. FERTILITY AND STERILITY, 100, 1236-1240.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/93304
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