The aim of present study is to investigate the immunohistochemical identification of cNOS-containing prostatic structures in various species and in hyperplastic human prostate. We used specimens of rat, guinea pig and hamster. Human prostatic specimens were removed from patients suffering of prostatic hyperplasia. The study was processed by ABC method. Immunohistochemical staining with anti nNOS antibody revealed the existence of strong reactivity in numerous ganglionic cell bodies and varicose nerve fibers in the fibromuscular stroma, in smooth cells around acini and in blood vessels endothelium. Surprisingly nNOS immunoreactivity appears in the glandular epithelium. In the hyperplastic prostate the nitroxidergic innervation of stroma and glandular epithelium is almost absent. These data provide a morphological evidence for a segmental differentiation of NOS-containing prostatic structures and consequently suggest that in the prostate NO plays selective neural and paraneural roles in the control of blood flow, smooth-muscle tone (relaxation) and secretory function. Further immunohistochemical studies are carrying out to elucidate the prostatic role in aging, after hormonal perturbation and pathological processes.

BONAVENTURA, G., TESSITORE, V., SPATOLA, G.F., UZZO, M.L., GERBINO, A. (2007). Immunohistochemical Expression of NO-synthase Constitutive Isoform (cNOS)in Mammals Normal Prostate and in Human Prostatic Obstructive Hyperplasia. RESEARCH JOURNAL OF BIOLOGICAL SCIENCES, 340-343.

Immunohistochemical Expression of NO-synthase Constitutive Isoform (cNOS)in Mammals Normal Prostate and in Human Prostatic Obstructive Hyperplasia

BONAVENTURA, Giuseppe;TESSITORE, Vincenzo;SPATOLA, Giovanni Francesco;UZZO, Maria Laura;GERBINO, Aldo
2007-01-01

Abstract

The aim of present study is to investigate the immunohistochemical identification of cNOS-containing prostatic structures in various species and in hyperplastic human prostate. We used specimens of rat, guinea pig and hamster. Human prostatic specimens were removed from patients suffering of prostatic hyperplasia. The study was processed by ABC method. Immunohistochemical staining with anti nNOS antibody revealed the existence of strong reactivity in numerous ganglionic cell bodies and varicose nerve fibers in the fibromuscular stroma, in smooth cells around acini and in blood vessels endothelium. Surprisingly nNOS immunoreactivity appears in the glandular epithelium. In the hyperplastic prostate the nitroxidergic innervation of stroma and glandular epithelium is almost absent. These data provide a morphological evidence for a segmental differentiation of NOS-containing prostatic structures and consequently suggest that in the prostate NO plays selective neural and paraneural roles in the control of blood flow, smooth-muscle tone (relaxation) and secretory function. Further immunohistochemical studies are carrying out to elucidate the prostatic role in aging, after hormonal perturbation and pathological processes.
2007
Settore BIO/17 - Istologia
BONAVENTURA, G., TESSITORE, V., SPATOLA, G.F., UZZO, M.L., GERBINO, A. (2007). Immunohistochemical Expression of NO-synthase Constitutive Isoform (cNOS)in Mammals Normal Prostate and in Human Prostatic Obstructive Hyperplasia. RESEARCH JOURNAL OF BIOLOGICAL SCIENCES, 340-343.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/9211
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact