The aim of the present study was to evaluate whether the cold-water extract of Pleurotus eryngii var. ferulae (CWE-Pef) and Pleurotus nebrodensis (CWE-Pn), two of the most prized wild and cultivated edible mushrooms can affect the tumour phenotype of human colon cancer HCT116 cells. Our results showed that treatment with CWE-Pef and CWE-Pn resulted in a significant inhibition of the viability of HCT116 cells and promoted apoptosis as also demonstrated by the increase of bax/bcl-2 mRNA ratio. Moreover, we observed that both extracts were able to inhibit cell migration and to affect homotypic and heterotypic cell-cell adhesion. It was also found that treatment with CWE-Pef and CWE-Pn negatively modulated the protein tyrosine phosphorylation as well as the phosphorylation levels of ERK1/2. In conclusion, the in vitro antitumor effects of CWE-Pef and CWE-Pn indicate that they can be considered as possible sources for new alternative therapeutic agents for cancer treatment.
Fontana, S., FLUGY PAPE', A., Schillaci, O., Cannizzaro, A., Gargano, M., Saitta, A., et al. (2014). In Vitro Antitumor Effects of the Cold-Water Extracts of Mediterranean Species of Genus Pleurotus (Higher Basidiomycetes) on Human Colon Cancer Cells. INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS, 16, 49-63 [10.1615/IntJMedMushr.v16.i1.50].
In Vitro Antitumor Effects of the Cold-Water Extracts of Mediterranean Species of Genus Pleurotus (Higher Basidiomycetes) on Human Colon Cancer Cells
FONTANA, Simona;FLUGY PAPE', Anna Maria;GARGANO, Maria Letizia;SAITTA, Alessandro;DE LEO, Giacomo;VENTURELLA, Giuseppe;ALESSANDRO, Riccardo
2014-01-01
Abstract
The aim of the present study was to evaluate whether the cold-water extract of Pleurotus eryngii var. ferulae (CWE-Pef) and Pleurotus nebrodensis (CWE-Pn), two of the most prized wild and cultivated edible mushrooms can affect the tumour phenotype of human colon cancer HCT116 cells. Our results showed that treatment with CWE-Pef and CWE-Pn resulted in a significant inhibition of the viability of HCT116 cells and promoted apoptosis as also demonstrated by the increase of bax/bcl-2 mRNA ratio. Moreover, we observed that both extracts were able to inhibit cell migration and to affect homotypic and heterotypic cell-cell adhesion. It was also found that treatment with CWE-Pef and CWE-Pn negatively modulated the protein tyrosine phosphorylation as well as the phosphorylation levels of ERK1/2. In conclusion, the in vitro antitumor effects of CWE-Pef and CWE-Pn indicate that they can be considered as possible sources for new alternative therapeutic agents for cancer treatment.
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