Archivio istituzionale della ricerca dell'Università degli Studi di Palermo
IRIS è il sistema di gestione integrata dei dati della ricerca (persone, pubblicazioni, progetti, attività) adottato dall’Università di Palermo, e ha lo scopo di raccogliere, conservare, documentare, e diffondere ad accesso aperto le informazioni relative alla produzione scientifica degli autori afferenti all’Ateneo.
EnglishIn this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully cryopreserved using the droplet-vitrification technique. Growth recovery of cryopreserved shoot tips was possible only when samples were pretreated for 16 h in liquid medium with 0.3Msucrose, then for 5 h in liquid medium with 0.7M sucrose before performing the cryopreservation protocol. Optimal conditions included treatment for 20 min in a loading solution containing 1.9M glycerol + 0.5M sucrose, treatment with vitrification solution B5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90 min or vitrification solution A9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20 min, rapid cooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20 min in unloading solution containing 1.2M sucrose. Under these conditions, 37% recovery of cryopreserved shoot tips was achieved. Regrowth of cryopreserved samples was slow but always direct, without callus formation.
Barraco, G., Sylvestre, I., Iapichino, G., & Engelmann, F. (2011). Cryopreservation of Limonium serotinum apical meristems from in vitro plantlets using droplet-vitrification. SCIENTIA HORTICULTURAE, 130, 309-313.
| Data di pubblicazione: | 2011 |
| Titolo: | Cryopreservation of Limonium serotinum apical meristems from in vitro plantlets using droplet-vitrification |
| Autori: | |
| Citazione: | Barraco, G., Sylvestre, I., Iapichino, G., & Engelmann, F. (2011). Cryopreservation of Limonium serotinum apical meristems from in vitro plantlets using droplet-vitrification. SCIENTIA HORTICULTURAE, 130, 309-313. |
| Rivista: | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.scienta.2011.07.001 |
| Abstract: | In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully cryopreserved using the droplet-vitrification technique. Growth recovery of cryopreserved shoot tips was possible only when samples were pretreated for 16 h in liquid medium with 0.3Msucrose, then for 5 h in liquid medium with 0.7M sucrose before performing the cryopreservation protocol. Optimal conditions included treatment for 20 min in a loading solution containing 1.9M glycerol + 0.5M sucrose, treatment with vitrification solution B5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90 min or vitrification solution A9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20 min, rapid cooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20 min in unloading solution containing 1.2M sucrose. Under these conditions, 37% recovery of cryopreserved shoot tips was achieved. Regrowth of cryopreserved samples was slow but always direct, without callus formation. |
| Settore Scientifico Disciplinare: | Settore AGR/04 - Orticoltura E Floricoltura |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
File in questo prodotto:
| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| Cryopreservation of Limonium serotinum apical meristems from in vitro plantlets.pdf | N/A | Administrator Richiedi una copia |
http://hdl.handle.net/10447/79130
Copyright © 2020