In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully cryopreserved using the droplet-vitrification technique. Growth recovery of cryopreserved shoot tips was possible only when samples were pretreated for 16 h in liquid medium with 0.3Msucrose, then for 5 h in liquid medium with 0.7M sucrose before performing the cryopreservation protocol. Optimal conditions included treatment for 20 min in a loading solution containing 1.9M glycerol + 0.5M sucrose, treatment with vitrification solution B5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90 min or vitrification solution A9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20 min, rapid cooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20 min in unloading solution containing 1.2M sucrose. Under these conditions, 37% recovery of cryopreserved shoot tips was achieved. Regrowth of cryopreserved samples was slow but always direct, without callus formation.
Barraco, G., Sylvestre, I., Iapichino, G., & Engelmann, F. (2011). Cryopreservation of Limonium serotinum apical meristems from in vitro plantlets using droplet-vitrification. SCIENTIA HORTICULTURAE, 130, 309-313 [10.1016/j.scienta.2011.07.001].
Data di pubblicazione: | 2011 | |
Titolo: | Cryopreservation of Limonium serotinum apical meristems from in vitro plantlets using droplet-vitrification | |
Autori: | ||
Citazione: | Barraco, G., Sylvestre, I., Iapichino, G., & Engelmann, F. (2011). Cryopreservation of Limonium serotinum apical meristems from in vitro plantlets using droplet-vitrification. SCIENTIA HORTICULTURAE, 130, 309-313 [10.1016/j.scienta.2011.07.001]. | |
Rivista: | ||
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.scienta.2011.07.001 | |
Abstract: | In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully cryopreserved using the droplet-vitrification technique. Growth recovery of cryopreserved shoot tips was possible only when samples were pretreated for 16 h in liquid medium with 0.3Msucrose, then for 5 h in liquid medium with 0.7M sucrose before performing the cryopreservation protocol. Optimal conditions included treatment for 20 min in a loading solution containing 1.9M glycerol + 0.5M sucrose, treatment with vitrification solution B5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90 min or vitrification solution A9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20 min, rapid cooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20 min in unloading solution containing 1.2M sucrose. Under these conditions, 37% recovery of cryopreserved shoot tips was achieved. Regrowth of cryopreserved samples was slow but always direct, without callus formation. | |
Settore Scientifico Disciplinare: | Settore AGR/04 - Orticoltura E Floricoltura | |
Appare nelle tipologie: | 1.01 Articolo in rivista |
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