Abstract PURPOSE: An observational clinical and molecular study was designed to evaluate the effects of the administration of recombinant human FSH on sperm DNA fragmentation in men with a non-classical form of hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia. METHODS: In the study were included 53 men with a non-classical form of hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia. In all patients, sperm DNA fragmentation index (DFI), assessed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) in situ DNA nick end-labelling (TUNEL) assay, was evaluated before starting the treatment with 150 IU of recombinant human FSH, given three times a week for at least 3 months. Patients' semen analysis and DNA fragmentation index were re-evaluated after the 3-month treatment period. RESULTS: After recombinant human FSH therapy, we did not find any differences in terms of sperm count, motility and morphology. The average DNA fragmentation index was significantly reduced (21.15 vs 15.2, p < 0.05), but we found a significant reduction in patients with high basal DFI values (>15 %), while no significant variation occurred in the patients with DFI values ≤15 %. CONCLUSIONS: Recombinant human FSH administration improves sperm DNA integrity in hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia men with DNA fragmentation index value >15 % .

Ruvolo, G., Roccheri, M.C., Brucculeri, A.M., Longobardi, S., Cittadini, E., & Bosco, L. (2013). Lower sperm DNA fragmentation after r-FSH administration in functional hypogonadotropic hypogonadism. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 30(4), 497-503 [10.1007/s10815-013-9951-y].

Lower sperm DNA fragmentation after r-FSH administration in functional hypogonadotropic hypogonadism

ROCCHERI, Maria Carmela;BOSCO, Liana
2013

Abstract

Abstract PURPOSE: An observational clinical and molecular study was designed to evaluate the effects of the administration of recombinant human FSH on sperm DNA fragmentation in men with a non-classical form of hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia. METHODS: In the study were included 53 men with a non-classical form of hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia. In all patients, sperm DNA fragmentation index (DFI), assessed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) in situ DNA nick end-labelling (TUNEL) assay, was evaluated before starting the treatment with 150 IU of recombinant human FSH, given three times a week for at least 3 months. Patients' semen analysis and DNA fragmentation index were re-evaluated after the 3-month treatment period. RESULTS: After recombinant human FSH therapy, we did not find any differences in terms of sperm count, motility and morphology. The average DNA fragmentation index was significantly reduced (21.15 vs 15.2, p < 0.05), but we found a significant reduction in patients with high basal DFI values (>15 %), while no significant variation occurred in the patients with DFI values ≤15 %. CONCLUSIONS: Recombinant human FSH administration improves sperm DNA integrity in hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia men with DNA fragmentation index value >15 % .
Settore BIO/06 - Anatomia Comparata E Citologia
http://link.springer.com/article/10.1007%2Fs10815-013-9951-y
Ruvolo, G., Roccheri, M.C., Brucculeri, A.M., Longobardi, S., Cittadini, E., & Bosco, L. (2013). Lower sperm DNA fragmentation after r-FSH administration in functional hypogonadotropic hypogonadism. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 30(4), 497-503 [10.1007/s10815-013-9951-y].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/76262
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