Brain distribution pattern of “street” heroin metabolites (morphine and codeine) was investigated in two fatalities due to “acute narcotism”. A suitable sample pretreatment prior to solid-phase-extraction was developed to achieve a good recovery of the analytes and to eliminate the interfering species. After derivatization with MSTFA, samples were analyzed by GC/MS. Specificity, accuracy, precision and linearity of the method were evaluated; LOD and LOQ were, respectively, 10 ng/25 ng for morphine and 5 ng/10 ng for codeine. This method was applied to the analysis of six brain areas (hippocampus, frontal lobe, occipital lobe, nuclei, bulb and pons) coming from two cases of heroin-related deaths. No evidence of accumulation of metabolites in a specific brain region was found

Guerrini, K., Argo, A., Borroni, C., Catalano, D., Dell’Acqua, L., Farè, F., et al. (2012). Development and validation of a reliable method for studying the distribution pattern for opiates metabolites in brain. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 73(special issue), 125-130 [10.1016/j.jpba.2012.04.001].

Development and validation of a reliable method for studying the distribution pattern for opiates metabolites in brain

ARGO, Antonina;PROCACCIANTI, Paolo;
2012-01-01

Abstract

Brain distribution pattern of “street” heroin metabolites (morphine and codeine) was investigated in two fatalities due to “acute narcotism”. A suitable sample pretreatment prior to solid-phase-extraction was developed to achieve a good recovery of the analytes and to eliminate the interfering species. After derivatization with MSTFA, samples were analyzed by GC/MS. Specificity, accuracy, precision and linearity of the method were evaluated; LOD and LOQ were, respectively, 10 ng/25 ng for morphine and 5 ng/10 ng for codeine. This method was applied to the analysis of six brain areas (hippocampus, frontal lobe, occipital lobe, nuclei, bulb and pons) coming from two cases of heroin-related deaths. No evidence of accumulation of metabolites in a specific brain region was found
2012
Settore MED/43 - Medicina Legale
Guerrini, K., Argo, A., Borroni, C., Catalano, D., Dell’Acqua, L., Farè, F., et al. (2012). Development and validation of a reliable method for studying the distribution pattern for opiates metabolites in brain. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 73(special issue), 125-130 [10.1016/j.jpba.2012.04.001].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/75893
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