Dual targeted drug delivery systems represent a potential platform for developing efficient vector to tumor sites. In this study we evaluated a folate- and magnetic-targeted nanocarriers based on 10 nm iron oxide nanodomais coated with the properly synthesized and characterized folic acid (FA)-functionalized amphiphilic copolymer PHEA-PLA-PEG-FA. FA was chemically conjugated to one end of diamino-polyethylene glycol of 2000 Da, in order to ensure its exposition on the polymer coated magnetic nanoparticles (MNPs-FA). The prepared nanoparticles have been exhaustively characterized by different methods, including DLS, SEM, FT-IR and magnetic measurements. Magnetic nanoparticles showed dimension of about 37 nm with a narrow size distribution and a characteristic superparamagnetic behaviour. The lack of cytotoxicity of MNPs-FA and MNPs was assessed both on MCF7 cells, used as a model tumor cell line, and on 16HBE, used as normal human cell model, by evaluating cell viability using MTS assay, while the preferential internalization of MNPs-FA into tumor cells rather that into normal cells was confirmed by the quantization of internalized iron oxide. Uptake studies were also performed in the presence of a permanent magnet in order to verify the synergistic effect of magnetic field in enhancing the internalization of magnetic nanoparticles. Finally, real-time confocal microscopy experiments were carried out to further confirmed that FA ligand enhances the MNPs-FA accumulation into cancer cell cytoplasm.
|Data di pubblicazione:||2013|
|Titolo:||Cell Uptake Enhancement of Folate Targeted Polymer Coated Magnetic Nanoparticles|
|Autori:||Licciardi, M; Scialabba, C; Cavallaro, G; Sangregorio, C; Fantechi, E; Giammona, G|
|Tipologia:||Articolo su rivista|
|Citazione:||Licciardi, M., Scialabba, C., Cavallaro, G., Sangregorio, C., Fantechi, E., & Giammona, G. (2013). Cell Uptake Enhancement of Folate Targeted Polymer Coated Magnetic Nanoparticles. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 9, 949-964.|
|Tipo:||Articolo in rivista|
|Appare nelle tipologie:||01 - Articolo su rivista|