T lymphocytes are often induced naturally in melanoma patients and infiltrate tumors. Given that gamma delta T cells mediate antigen-specific killing of tumor cells, we studied the representation and the in vitro cytokine production and cytotoxic activity of tumor infiltrating gamma delta T cells from 74 patients with primary melanoma. We found that gamma delta T cells represent the major lymphocyte population infiltrating melanoma, and both V delta 1(+) and V delta 2(+) cells are involved. The majority of melanoma-infiltrating gamma delta cells showed effector memory and terminally-differentiated phenotypes and, accordingly, polyclonal gamma delta T cell lines obtained from tumor-infiltrating immune cells produced IFN-gamma and TNF-alpha and were capable of killing melanoma cell lines in vitro. The cytotoxic capability of V delta 2 cell lines was further improved by pre-treatment of tumor target cells with zoledronate. Moreover, higher rate of gamma delta T cells isolation and percentages of V delta 2 cells correlate with early stage of development of melanoma and absence of metastasis. Altogether, our results suggest that a natural immune response mediated by gamma delta T lymphocytes may contribute to the immunosurveillance of melanoma.

Cordova, A., Toia, F., La Mendola, C., Orlando, V., Meraviglia, S., Rinaldi, G., et al. (2012). Characterization of human γδ T lymphocytes infiltrating primary malignant melanomas. PLOS ONE, 7(11) [10.1371/journal.pone.0049878].

Characterization of human γδ T lymphocytes infiltrating primary malignant melanomas

CORDOVA, Adriana;TOIA, Francesca;LA MENDOLA, Carmela;ORLANDO, Valentina;MERAVIGLIA, Serena;RINALDI, Gaetana;TODARO, Matilde;CICERO, Giuseppe;LI DONNI, Paolo;CACCAMO, Nadia Rosalia;STASSI, Giorgio;DIELI, Francesco;MOSCHELLA, Francesco
2012-01-01

Abstract

T lymphocytes are often induced naturally in melanoma patients and infiltrate tumors. Given that gamma delta T cells mediate antigen-specific killing of tumor cells, we studied the representation and the in vitro cytokine production and cytotoxic activity of tumor infiltrating gamma delta T cells from 74 patients with primary melanoma. We found that gamma delta T cells represent the major lymphocyte population infiltrating melanoma, and both V delta 1(+) and V delta 2(+) cells are involved. The majority of melanoma-infiltrating gamma delta cells showed effector memory and terminally-differentiated phenotypes and, accordingly, polyclonal gamma delta T cell lines obtained from tumor-infiltrating immune cells produced IFN-gamma and TNF-alpha and were capable of killing melanoma cell lines in vitro. The cytotoxic capability of V delta 2 cell lines was further improved by pre-treatment of tumor target cells with zoledronate. Moreover, higher rate of gamma delta T cells isolation and percentages of V delta 2 cells correlate with early stage of development of melanoma and absence of metastasis. Altogether, our results suggest that a natural immune response mediated by gamma delta T lymphocytes may contribute to the immunosurveillance of melanoma.
2012
Settore MED/04 - Patologia Generale
Settore MED/19 - Chirurgia Plastica
Cordova, A., Toia, F., La Mendola, C., Orlando, V., Meraviglia, S., Rinaldi, G., et al. (2012). Characterization of human γδ T lymphocytes infiltrating primary malignant melanomas. PLOS ONE, 7(11) [10.1371/journal.pone.0049878].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/74545
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