A method to assess the expansion of antigen-specific intracellular IFN-c positive T cell subsets during the infection will be helpful for a better understanding of mycoplasmal infections physiopathology in the sheep. We analysed the percentage of antigen-specific lymphocytes positive for intracellular IFN-c during the infection of sheep with Mycoplasma agalactiae by culturing peripheral blood mononuclear cells of infected or uninfected animals with irradiated M. agalactiae. The expansion of antigen-specific IFN-c positive lymphocytes in infected sheep was initially sustained by CD4+ T cells at day 15 after infection, when antigen specific IgG start to be detectable, followed by CD8/IFN-c double positive cells. cd T-cells were not expanded at any time point analysed. IFNc+ T cells disappear 60 days after infection, suggesting that antigen specific IFNc+ T cells, mainly detected in the early phase of the disease, could be useful to understand the role of cell-mediated immunity during M. agalactiae infection.

La Manna, M.P., Agnone, A., Villari, S., Puleio, R., Vitale, M., Nicholas, R., et al. (2011). Expansion of intracellular IFN-g positive lymphocytes during Mycoplasma agalactiae infection in sheep. RESEARCH IN VETERINARY SCIENCE, 91(3), 64-67 [10.1016/j.rvsc.2011.01.029].

Expansion of intracellular IFN-g positive lymphocytes during Mycoplasma agalactiae infection in sheep

LA MANNA, Marco Pio;AGNONE, Annalisa;SIRECI, Guido;DIELI, Francesco;
2011

Abstract

A method to assess the expansion of antigen-specific intracellular IFN-c positive T cell subsets during the infection will be helpful for a better understanding of mycoplasmal infections physiopathology in the sheep. We analysed the percentage of antigen-specific lymphocytes positive for intracellular IFN-c during the infection of sheep with Mycoplasma agalactiae by culturing peripheral blood mononuclear cells of infected or uninfected animals with irradiated M. agalactiae. The expansion of antigen-specific IFN-c positive lymphocytes in infected sheep was initially sustained by CD4+ T cells at day 15 after infection, when antigen specific IgG start to be detectable, followed by CD8/IFN-c double positive cells. cd T-cells were not expanded at any time point analysed. IFNc+ T cells disappear 60 days after infection, suggesting that antigen specific IFNc+ T cells, mainly detected in the early phase of the disease, could be useful to understand the role of cell-mediated immunity during M. agalactiae infection.
La Manna, M.P., Agnone, A., Villari, S., Puleio, R., Vitale, M., Nicholas, R., et al. (2011). Expansion of intracellular IFN-g positive lymphocytes during Mycoplasma agalactiae infection in sheep. RESEARCH IN VETERINARY SCIENCE, 91(3), 64-67 [10.1016/j.rvsc.2011.01.029].
File in questo prodotto:
File Dimensione Formato  
4- Expansion of intracellular IFN-γ positive lymphocytes during Mycoplasma agalactiae infection in sheep..pdf

non disponibili

Descrizione: Articolo principale
Dimensione 392.1 kB
Formato Adobe PDF
392.1 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/73507
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 6
social impact