Over the past few decades, microRNAs (miRNAs) have gained significant attention for their role in regulating gene expression and their association with various diseases, including cancer. These small noncoding RNAs (sncRNAs) have been identified as crucial regulators of different biological functions. However, recent advancements in high-throughput sequencing technologies have revealed a more complex miRNAome, driven by phenomena such as RNA editing and isomiRs, which modify miRNA sequences. This increasing complexity has necessitated the development of new tools to analyze the miRNAome and better understand their biological roles. To address this challenge, isoTar was developed—a high-performance, web-based containerized application designed for miRNA consensus-targeting prediction and functional enrichment analyses. This chapter provides a comprehensive overview of isoTar (https://ncrnaome.osumc.edu/isotar/), including benchmarks of its performance and a guide to its usage.
Di Bella, S., Orilio, F., Gu, S., Distefano, R., Liu, J., Gaggianesi, M., et al. (2026). isoTar: miRNA-Target Consensus Prediction with Enrichment Analysis for Canonical and Modified miRNAs. In G.N. Alessandro Laganà (a cura di), MicroRNA Target Identification: Methods and Protocols (pp. 61-85). Humana Press Inc. [10.1007/978-1-0716-5392-0_5].
isoTar: miRNA-Target Consensus Prediction with Enrichment Analysis for Canonical and Modified miRNAs
Di Bella S.;Orilio F.;Distefano R.;Liu J.;Gaggianesi M.;Brancaccio R. N.;
2026-01-01
Abstract
Over the past few decades, microRNAs (miRNAs) have gained significant attention for their role in regulating gene expression and their association with various diseases, including cancer. These small noncoding RNAs (sncRNAs) have been identified as crucial regulators of different biological functions. However, recent advancements in high-throughput sequencing technologies have revealed a more complex miRNAome, driven by phenomena such as RNA editing and isomiRs, which modify miRNA sequences. This increasing complexity has necessitated the development of new tools to analyze the miRNAome and better understand their biological roles. To address this challenge, isoTar was developed—a high-performance, web-based containerized application designed for miRNA consensus-targeting prediction and functional enrichment analyses. This chapter provides a comprehensive overview of isoTar (https://ncrnaome.osumc.edu/isotar/), including benchmarks of its performance and a guide to its usage.
| File | Dimensione | Formato | |
|---|---|---|---|
|
Cap05_isoTar_miRNA-Target_Consensus_Prediction.pdf
Solo gestori archvio
Tipologia:
Versione Editoriale
Dimensione
3.61 MB
Formato
Adobe PDF
|
3.61 MB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
