Upgrading existing wastewater treatment plants to achieve higher efficiency requires compact process-intensification strategies, particularly where the construction of additional treatment units is constrained by limited land availability. This study evaluated a compact pilot-scale integrated fixed-film activated sludge (IFAS) membrane photobioreactor treating real municipal wastewater under low carbon-to-nitrogen (C/N) conditions. The system combined suspended activated sludge, attached biofilm, microalgae ( Chlorella sp. ) and submerged membrane filtration. Two operating periods were investigated by increasing the algae:sludge mass ratio from 1:5–1:3. The reactor achieved high carbon removal, with total chemical oxygen demand (COD) removal improving from 91.7 ± 4.3% to 95.9 ± 1.6% despite the higher influent organic load in the second period (1.5 ± 0.8 kg COD/m3 d). Ammonium removal also increased from 82.2 ± 26.4% to 96.5 ± 5.5%, indicating enhanced nitrification capacity. In contrast, total nitrogen (TN) removal remained moderate, decreasing from 49.5 ± 23.6% to 39.8 ± 21.4% as carbon availability declined (C/N in period 2: 1.2 g soluble COD/TN), confirming carbon limitation as the main bottleneck for complete nitrogen removal. Phosphate removal was poor overall, although a modest improvement was observed at a higher algal fraction. Biomass characterisation showed greater biofilm accumulation and algal proliferation in the second period, with attached biomass doubling and chlorophyll-a increasing from 2.7 ± 0.2–4.3 ± 1.5 mg/L. Overall, the results demonstrate the feasibility of this IFAS membrane photobioreactor as an advanced proof of concept for low-COD/N wastewater treatment, while highlighting the need for further optimisation to improve denitrification and phosphorus removal.
Mineo, A., Scargiali, F., Lima, S., Mannina, G. (2026). Integrated fixed film activated sludge and algae system for advanced membrane-based wastewater treatment: A pilot plant experiment with real wastewater. BIOCHEMICAL ENGINEERING JOURNAL, 236 [10.1016/j.bej.2026.110369].
Integrated fixed film activated sludge and algae system for advanced membrane-based wastewater treatment: A pilot plant experiment with real wastewater
Mineo, Antonio
Primo
;Scargiali, Francesca;Lima, Serena;Mannina, Giorgio
2026-08-17
Abstract
Upgrading existing wastewater treatment plants to achieve higher efficiency requires compact process-intensification strategies, particularly where the construction of additional treatment units is constrained by limited land availability. This study evaluated a compact pilot-scale integrated fixed-film activated sludge (IFAS) membrane photobioreactor treating real municipal wastewater under low carbon-to-nitrogen (C/N) conditions. The system combined suspended activated sludge, attached biofilm, microalgae ( Chlorella sp. ) and submerged membrane filtration. Two operating periods were investigated by increasing the algae:sludge mass ratio from 1:5–1:3. The reactor achieved high carbon removal, with total chemical oxygen demand (COD) removal improving from 91.7 ± 4.3% to 95.9 ± 1.6% despite the higher influent organic load in the second period (1.5 ± 0.8 kg COD/m3 d). Ammonium removal also increased from 82.2 ± 26.4% to 96.5 ± 5.5%, indicating enhanced nitrification capacity. In contrast, total nitrogen (TN) removal remained moderate, decreasing from 49.5 ± 23.6% to 39.8 ± 21.4% as carbon availability declined (C/N in period 2: 1.2 g soluble COD/TN), confirming carbon limitation as the main bottleneck for complete nitrogen removal. Phosphate removal was poor overall, although a modest improvement was observed at a higher algal fraction. Biomass characterisation showed greater biofilm accumulation and algal proliferation in the second period, with attached biomass doubling and chlorophyll-a increasing from 2.7 ± 0.2–4.3 ± 1.5 mg/L. Overall, the results demonstrate the feasibility of this IFAS membrane photobioreactor as an advanced proof of concept for low-COD/N wastewater treatment, while highlighting the need for further optimisation to improve denitrification and phosphorus removal.| File | Dimensione | Formato | |
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