Fish trait-based indicators of mercury bioaccumulation under natural ocean acidification: Insights from a mediterranean CO₂ vent system

Ocean acidification is not an isolated climate-related threat to marine organisms, as it may act in combination with other stressors such as trace element contamination. Here, we took advantage of naturally acidified conditions and mercury spill-out from the shallow CO2 vent of Vulcano Island (Italy) to test whether fish functional traits explain contaminant dynamics better than species identity under ocean acidification scenarios. Specifically, we investigated if trophic group and mobility influence total mercury (THg) bioaccumulation and trophic transfer in fish inhabiting Cymodocea nodosa seagrass meadows under low and ambient pH conditions. Lowmobility benthic fish, mainly represented by invertebrate feeders and small piscivores, exhibited higher THg concentration at the vent site than at reference sites, while highly mobile zooplanktivorous fish showed no significant differences, highlighting the importance of fish mobility and local trophic benthic pathways in shaping contaminant exposure and bioaccumulation. Moreover, the relationship between trophic position and THg concentration in fish was stronger in the low pH site, indicating enhanced trophic transfer and biomagnification when Hg availability is higher. In contrast, weak or absent biomagnification in the reference sites suggests that Hg transfer depends more on contaminant availability and food web structure than trophic position alone. Overall, these findings indicate that functional traits better predict Hg bioaccumulation patterns than species identity, supporting trait-based approach to assess contaminant dynamics in future ocean acidification scenarios.