Background: The major allergens in Parietaria pollen, Par j 1 and Par j 2, have been identified as lipid transfer proteins. The family of the Par j 1 allergens is composed of two isoforms, which differ by the presence of a 37 amino acid peptide (Par37) exclusive to the Par j 1.0101 isoform. The goal of this study was to elucidate the biological properties of the Par37 peptide. Methods: In silico analysis, spectrofluorimetric experiments and in vitro cell culture assays were used to identify the biological properties of Par37. In addition, a mouse model of sensitization was used to study the influence of Par37 in the murine immune response. Results: In silico analysis predicted that Par37 displays characteristics of a host defence peptide. Spectrofluorimetric analysis, real-time PCR and ELISA assays demonstrated that Par37 possesses an LPS-binding activity influencing cell signalling in vitro. In RAW264.7 cells, LPS-induced IL-6 and TNF-a transcription and translation were inhibited after preincubation with Par37. Consistent with these data, inhibition of IFN-c secretion was observed in murine spleen cells and in human PBMC. Finally, mice immunized with the two Par j 1 isoforms differing in the presence or absence of the Par37 peptide showed different immunological behaviours in vivo. Conclusions: This study demonstrates that the Par j 1.0101 allergen displays LPSbinding activity due to the presence of a 37 amino acid COOH-terminal region and that this region is capable of influencing cytokine and antibody responses in vitro and in vivo.
|Data di pubblicazione:||2013|
|Titolo:||The major allergen of the Parietaria pollen contains an LPS-binding region with immuno-modulatory activity|
|Citazione:||Bonura, A., Corinti, S., Schiavi, E., Giacomazza, D., Gianguzza, F., Di Felice, G., et al. (2013). The major allergen of the Parietaria pollen contains an LPS-binding region with immuno-modulatory activity. ALLERGY, online.|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.111/all.12086|
|Settore Scientifico Disciplinare:||Settore BIO/11 - Biologia Molecolare|
|Appare nelle tipologie:||1.01 Articolo in rivista|