Vγ9Vδ2 T lymphocytes recognize nonpeptidic antigens without presentation by MHC molecules and display pleiotropic features. Here we report that coculture of Vγ9Vδ2 cells with phosphoantigen and IL-21 leads to selective expression of the transcription repressor Bcl-6 and polarization toward a lymphocyte subset displaying features of follicular B-helper T (T(FH)) cells. T(FH) like Vγ9Vδ2 cells have a predominant central memory (CD27(+)CD45RA(-)) phenotype and express ICOS, CD40L and CXCR5. Upon antigen activation, they secrete IL-4, IL-10 and CXCL13, and provide B-cell help for antibody production in vitro. Our findings delineate a subset of human Vγ9Vδ2 lymphocytes, which, upon interaction with IL-21-producing CD4 T(FH) cells and B cells in secondary lymphoid organs, is implicated in the production of high affinity antibodies against microbial pathogens.
|Data di pubblicazione:||2012|
|Titolo:||IL-21 regulates the differentiation of a human γδ T cell subset equipped with B cell helper activity.|
|Citazione:||Caccamo, N., Todaro, M., LA MANNA, M., Sireci, G., Stassi, G., & Dieli, F. (2012). IL-21 regulates the differentiation of a human γδ T cell subset equipped with B cell helper activity. PLOS ONE, 7(7), e41940.|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1371/journal.pone.0041940|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Appare nelle tipologie:||1.01 Articolo in rivista|