Vγ9Vδ2 T lymphocytes recognize nonpeptidic antigens without presentation by MHC molecules and display pleiotropic features. Here we report that coculture of Vγ9Vδ2 cells with phosphoantigen and IL-21 leads to selective expression of the transcription repressor Bcl-6 and polarization toward a lymphocyte subset displaying features of follicular B-helper T (T(FH)) cells. T(FH) like Vγ9Vδ2 cells have a predominant central memory (CD27(+)CD45RA(-)) phenotype and express ICOS, CD40L and CXCR5. Upon antigen activation, they secrete IL-4, IL-10 and CXCL13, and provide B-cell help for antibody production in vitro. Our findings delineate a subset of human Vγ9Vδ2 lymphocytes, which, upon interaction with IL-21-producing CD4 T(FH) cells and B cells in secondary lymphoid organs, is implicated in the production of high affinity antibodies against microbial pathogens.

Caccamo, N., Todaro, M., La Manna, M.P., Sireci, G., Stassi, G., Dieli, F. (2012). IL-21 regulates the differentiation of a human γδ T cell subset equipped with B cell helper activity. PLOS ONE, 7(7), e41940 [10.1371/journal.pone.0041940].

IL-21 regulates the differentiation of a human γδ T cell subset equipped with B cell helper activity.

CACCAMO, Nadia Rosalia;TODARO, Matilde;LA MANNA, Marco Pio;SIRECI, Guido;STASSI, Giorgio;DIELI, Francesco
2012-01-01

Abstract

Vγ9Vδ2 T lymphocytes recognize nonpeptidic antigens without presentation by MHC molecules and display pleiotropic features. Here we report that coculture of Vγ9Vδ2 cells with phosphoantigen and IL-21 leads to selective expression of the transcription repressor Bcl-6 and polarization toward a lymphocyte subset displaying features of follicular B-helper T (T(FH)) cells. T(FH) like Vγ9Vδ2 cells have a predominant central memory (CD27(+)CD45RA(-)) phenotype and express ICOS, CD40L and CXCR5. Upon antigen activation, they secrete IL-4, IL-10 and CXCL13, and provide B-cell help for antibody production in vitro. Our findings delineate a subset of human Vγ9Vδ2 lymphocytes, which, upon interaction with IL-21-producing CD4 T(FH) cells and B cells in secondary lymphoid organs, is implicated in the production of high affinity antibodies against microbial pathogens.
Settore MED/04 - Patologia Generale
Caccamo, N., Todaro, M., La Manna, M.P., Sireci, G., Stassi, G., Dieli, F. (2012). IL-21 regulates the differentiation of a human γδ T cell subset equipped with B cell helper activity. PLOS ONE, 7(7), e41940 [10.1371/journal.pone.0041940].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/65899
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