3D printing is an additive manufacturing technique that can transform 3D virtual models into physical objects by layer-by-layer deposition of materials that can undergo rapid liquid-to-solid transformation after dispensing. Hydrogels are one of the most interesting and challenging classes of material systems that can be employed as inks, and hydrogel-based 3D printing has been exploited in tissue engineering and regenerative medicine. Here, hydrogel bio-inks using k-Carrageenan (kC) and poly(vinyl alcohol) (PVA) have been formulated with the objective of tuning the printability properties of bio-ink. The kC allows the systems to undergo rapid sol-to-gel transitions upon cooling from 60 °C and above to body temperature, while the need to introduce PVA is related to optimizing the viscosity of the ink solution to enable 3D printing with a continuous filament and to introduce interconnected porosity in the scaffold. γ-radiation-induced chemical modification of kC polymer powder is successfully used here as a mean to induce noticeable modifications in the polymer molecular weight distribution and polymer structure and to optimize the printing properties of bio-ink formulations. The study aims to develop hydrogel formulations with viscoelastic properties and sol-gel transitions suitable for use as bioinks for 3D printing scaffolds with adipose stem cell spheroids for cartilage or bone tissue reconstruction.

Galvano S., Di Stefano A.B., Muscolino E., Trapani M., Toia F., Dispenza C. (2024). Development of Adaptable 3D-Bioprinted Scaffold for Tissue Regeneration. CHEMICAL ENGINEERING TRANSACTIONS, 110, 253-258 [10.3303/CET24110043].

Development of Adaptable 3D-Bioprinted Scaffold for Tissue Regeneration

Galvano S.
Primo
;
Di Stefano A. B.;Muscolino E.
;
Trapani M.;Toia F.;Dispenza C.
Ultimo
2024-07-30

Abstract

3D printing is an additive manufacturing technique that can transform 3D virtual models into physical objects by layer-by-layer deposition of materials that can undergo rapid liquid-to-solid transformation after dispensing. Hydrogels are one of the most interesting and challenging classes of material systems that can be employed as inks, and hydrogel-based 3D printing has been exploited in tissue engineering and regenerative medicine. Here, hydrogel bio-inks using k-Carrageenan (kC) and poly(vinyl alcohol) (PVA) have been formulated with the objective of tuning the printability properties of bio-ink. The kC allows the systems to undergo rapid sol-to-gel transitions upon cooling from 60 °C and above to body temperature, while the need to introduce PVA is related to optimizing the viscosity of the ink solution to enable 3D printing with a continuous filament and to introduce interconnected porosity in the scaffold. γ-radiation-induced chemical modification of kC polymer powder is successfully used here as a mean to induce noticeable modifications in the polymer molecular weight distribution and polymer structure and to optimize the printing properties of bio-ink formulations. The study aims to develop hydrogel formulations with viscoelastic properties and sol-gel transitions suitable for use as bioinks for 3D printing scaffolds with adipose stem cell spheroids for cartilage or bone tissue reconstruction.
30-lug-2024
Galvano S., Di Stefano A.B., Muscolino E., Trapani M., Toia F., Dispenza C. (2024). Development of Adaptable 3D-Bioprinted Scaffold for Tissue Regeneration. CHEMICAL ENGINEERING TRANSACTIONS, 110, 253-258 [10.3303/CET24110043].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/658153
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