Treatment of chick embryos in ovo with hydrocortisone-21-phosphate (a single dose of 150 micrograms) caused a marked reduction of retinal thymidine kinase activity 24 h later. The inhibitory effect was highest (65-70%) in 8-10-day-old embryos and declined with age, disappearing after day 15. It was accompanied by a reduction in thickness of the retinal layers. Adrenocorticotropic hormone (ACTH) treatment (10 micrograms daily for 2 days) also produced an age-dependent inhibitory effect on retinal thymidine kinase, whereas treatment with a single dose of 200 micrograms of metopirone, a compound that prevents the 11 beta-hydroxylation of steroid molecules in the adrenal glands, impeded the decrease in thymidine kinase activity that normally occurs in chick embryo retina after day 9 of development. In addition, metopirone prevented the inhibition exerted by ACTH on thymidine kinase activity but had no effect on the action of hydrocortisone.

VENTO, R., D'ANCONA, G., GIULIANO, M., TAIBI, G., & TESORIERE, G. (1987). Influence of hydrocortisone on chick embryo retina development. JOURNAL OF NEUROCHEMISTRY, 48(6), 1693-1698.

Influence of hydrocortisone on chick embryo retina development

VENTO, Renza;GIULIANO, Michela;TAIBI, Gennaro;
1987

Abstract

Treatment of chick embryos in ovo with hydrocortisone-21-phosphate (a single dose of 150 micrograms) caused a marked reduction of retinal thymidine kinase activity 24 h later. The inhibitory effect was highest (65-70%) in 8-10-day-old embryos and declined with age, disappearing after day 15. It was accompanied by a reduction in thickness of the retinal layers. Adrenocorticotropic hormone (ACTH) treatment (10 micrograms daily for 2 days) also produced an age-dependent inhibitory effect on retinal thymidine kinase, whereas treatment with a single dose of 200 micrograms of metopirone, a compound that prevents the 11 beta-hydroxylation of steroid molecules in the adrenal glands, impeded the decrease in thymidine kinase activity that normally occurs in chick embryo retina after day 9 of development. In addition, metopirone prevented the inhibition exerted by ACTH on thymidine kinase activity but had no effect on the action of hydrocortisone.
VENTO, R., D'ANCONA, G., GIULIANO, M., TAIBI, G., & TESORIERE, G. (1987). Influence of hydrocortisone on chick embryo retina development. JOURNAL OF NEUROCHEMISTRY, 48(6), 1693-1698.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/65758
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