Treatment of chick embryos in ovo with hydrocortisone-21-phosphate (a single dose of 150 micrograms) caused a marked reduction of retinal thymidine kinase activity 24 h later. The inhibitory effect was highest (65-70%) in 8-10-day-old embryos and declined with age, disappearing after day 15. It was accompanied by a reduction in thickness of the retinal layers. Adrenocorticotropic hormone (ACTH) treatment (10 micrograms daily for 2 days) also produced an age-dependent inhibitory effect on retinal thymidine kinase, whereas treatment with a single dose of 200 micrograms of metopirone, a compound that prevents the 11 beta-hydroxylation of steroid molecules in the adrenal glands, impeded the decrease in thymidine kinase activity that normally occurs in chick embryo retina after day 9 of development. In addition, metopirone prevented the inhibition exerted by ACTH on thymidine kinase activity but had no effect on the action of hydrocortisone.

VENTO, R., D'ANCONA, G., GIULIANO, M., TAIBI, G., TESORIERE, G. (1987). Influence of hydrocortisone on chick embryo retina development. JOURNAL OF NEUROCHEMISTRY, 48(6), 1693-1698.

Influence of hydrocortisone on chick embryo retina development

VENTO, Renza;GIULIANO, Michela;TAIBI, Gennaro;
1987-01-01

Abstract

Treatment of chick embryos in ovo with hydrocortisone-21-phosphate (a single dose of 150 micrograms) caused a marked reduction of retinal thymidine kinase activity 24 h later. The inhibitory effect was highest (65-70%) in 8-10-day-old embryos and declined with age, disappearing after day 15. It was accompanied by a reduction in thickness of the retinal layers. Adrenocorticotropic hormone (ACTH) treatment (10 micrograms daily for 2 days) also produced an age-dependent inhibitory effect on retinal thymidine kinase, whereas treatment with a single dose of 200 micrograms of metopirone, a compound that prevents the 11 beta-hydroxylation of steroid molecules in the adrenal glands, impeded the decrease in thymidine kinase activity that normally occurs in chick embryo retina after day 9 of development. In addition, metopirone prevented the inhibition exerted by ACTH on thymidine kinase activity but had no effect on the action of hydrocortisone.
1987
VENTO, R., D'ANCONA, G., GIULIANO, M., TAIBI, G., TESORIERE, G. (1987). Influence of hydrocortisone on chick embryo retina development. JOURNAL OF NEUROCHEMISTRY, 48(6), 1693-1698.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/65758
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