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EnglishFetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.
Tesoriere, G., Vento, R., Calvaruso, G., Schiavo, M., Giuliano, M., & Taibi, G. (1989). Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum. BRAIN RESEARCH. DEVELOPMENTAL BRAIN RESEARCH., 47(1), 19-25.
| Data di pubblicazione: | 1989 |
| Titolo: | Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum |
| Autori: | |
| Citazione: | Tesoriere, G., Vento, R., Calvaruso, G., Schiavo, M., Giuliano, M., & Taibi, G. (1989). Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum. BRAIN RESEARCH. DEVELOPMENTAL BRAIN RESEARCH., 47(1), 19-25. |
| Rivista: | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/0165-3806(89)90104-1 |
| Abstract: | Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium. |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
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