Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.
Tesoriere, G., Vento, R., Calvaruso, G., Schiavo, M.R., Giuliano, M., Taibi, G. (1989). Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum. BRAIN RESEARCH. DEVELOPMENTAL BRAIN RESEARCH., 47(1), 19-25 [10.1016/0165-3806(89)90104-1].
Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum
VENTO, Renza;CALVARUSO, Giuseppe;GIULIANO, Michela;TAIBI, Gennaro
1989-01-01
Abstract
Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.
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