Archivio istituzionale della ricerca dell'Università degli Studi di Palermo
IRIS è il sistema di gestione integrata dei dati della ricerca (persone, pubblicazioni, progetti, attività) adottato dall’Università di Palermo, e ha lo scopo di raccogliere, conservare, documentare, e diffondere ad accesso aperto le informazioni relative alla produzione scientifica degli autori afferenti all’Ateneo.
EnglishFetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.
Tesoriere, G., Vento, R., Calvaruso, G., Schiavo, M., Giuliano, M., & Taibi, G. (1989). Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum. BRAIN RESEARCH. DEVELOPMENTAL BRAIN RESEARCH., 47(1), 19-25 [10.1016/0165-3806(89)90104-1].
| Data di pubblicazione: | 1989 | |
| Titolo: | Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum | |
| Autori: | ||
| Citazione: | Tesoriere, G., Vento, R., Calvaruso, G., Schiavo, M., Giuliano, M., & Taibi, G. (1989). Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum. BRAIN RESEARCH. DEVELOPMENTAL BRAIN RESEARCH., 47(1), 19-25 [10.1016/0165-3806(89)90104-1]. | |
| Rivista: | ||
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/0165-3806(89)90104-1 | |
| Abstract: | Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium. | |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
File in questo prodotto:
Copyright © 2022