Retinas of chick embryos contain insulin and further, are capable of synthesizing it, as demonstrated by incubating retinas at different ages (7th-18th day) with [3H]leucine. The synthesized radioactive insulin was isolated and assayed by means of a HPLC procedure. The synthesis of insulin was found to be highest in the youngest retinas studied (day 7), afterwards it declined with age except for an increment found at 14-15 day. Explants of chick embryo retinas, cultured in vitro, rapidly degraded insulin. Nevertheless, the content of immunoreactive insulin in retinal explants diminished slowly with the age of culture, so that, after 8 days of incubation, it was about 60% of the content found in the retinas at the beginning of incubation. This was proof that cultured explants are capable of efficiently synthesizing insulin. The synthesized [3H]insulin was released from explants into the medium. This was evident also after 6-8 days in culture.

TESORIERE, G., CALVARUSO, G., VENTO, R., GIULIANO, M., LAURICELLA, M., CARABILLO, M. (1994). Insulin synthesis in chick embryo retinas during development. NEUROCHEMICAL RESEARCH, 19(7), 821-825 [10.1007/BF00967450].

Insulin synthesis in chick embryo retinas during development

CALVARUSO, Giuseppe;VENTO, Renza;GIULIANO, Michela;LAURICELLA, Marianna;
1994-01-01

Abstract

Retinas of chick embryos contain insulin and further, are capable of synthesizing it, as demonstrated by incubating retinas at different ages (7th-18th day) with [3H]leucine. The synthesized radioactive insulin was isolated and assayed by means of a HPLC procedure. The synthesis of insulin was found to be highest in the youngest retinas studied (day 7), afterwards it declined with age except for an increment found at 14-15 day. Explants of chick embryo retinas, cultured in vitro, rapidly degraded insulin. Nevertheless, the content of immunoreactive insulin in retinal explants diminished slowly with the age of culture, so that, after 8 days of incubation, it was about 60% of the content found in the retinas at the beginning of incubation. This was proof that cultured explants are capable of efficiently synthesizing insulin. The synthesized [3H]insulin was released from explants into the medium. This was evident also after 6-8 days in culture.
TESORIERE, G., CALVARUSO, G., VENTO, R., GIULIANO, M., LAURICELLA, M., CARABILLO, M. (1994). Insulin synthesis in chick embryo retinas during development. NEUROCHEMICAL RESEARCH, 19(7), 821-825 [10.1007/BF00967450].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/65738
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