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EnglishRetinas of chick embryos contain insulin and further, are capable of synthesizing it, as demonstrated by incubating retinas at different ages (7th-18th day) with [3H]leucine. The synthesized radioactive insulin was isolated and assayed by means of a HPLC procedure. The synthesis of insulin was found to be highest in the youngest retinas studied (day 7), afterwards it declined with age except for an increment found at 14-15 day. Explants of chick embryo retinas, cultured in vitro, rapidly degraded insulin. Nevertheless, the content of immunoreactive insulin in retinal explants diminished slowly with the age of culture, so that, after 8 days of incubation, it was about 60% of the content found in the retinas at the beginning of incubation. This was proof that cultured explants are capable of efficiently synthesizing insulin. The synthesized [3H]insulin was released from explants into the medium. This was evident also after 6-8 days in culture.
Tesoriere, G., Calvaruso, G., Vento, R., Giuliano, M., Lauricella, M., & Carabillo, M. (1994). Insulin synthesis in chick embryo retinas during development. NEUROCHEMICAL RESEARCH, 19(7), 821-825.
| Data di pubblicazione: | 1994 |
| Titolo: | Insulin synthesis in chick embryo retinas during development |
| Autori: | |
| Citazione: | Tesoriere, G., Calvaruso, G., Vento, R., Giuliano, M., Lauricella, M., & Carabillo, M. (1994). Insulin synthesis in chick embryo retinas during development. NEUROCHEMICAL RESEARCH, 19(7), 821-825. |
| Rivista: | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1007/BF00967450 |
| Abstract: | Retinas of chick embryos contain insulin and further, are capable of synthesizing it, as demonstrated by incubating retinas at different ages (7th-18th day) with [3H]leucine. The synthesized radioactive insulin was isolated and assayed by means of a HPLC procedure. The synthesis of insulin was found to be highest in the youngest retinas studied (day 7), afterwards it declined with age except for an increment found at 14-15 day. Explants of chick embryo retinas, cultured in vitro, rapidly degraded insulin. Nevertheless, the content of immunoreactive insulin in retinal explants diminished slowly with the age of culture, so that, after 8 days of incubation, it was about 60% of the content found in the retinas at the beginning of incubation. This was proof that cultured explants are capable of efficiently synthesizing insulin. The synthesized [3H]insulin was released from explants into the medium. This was evident also after 6-8 days in culture. |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
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