Preparation of protein nuclear extracts is often the first step to study in vitro biological processes occurring in the nucleus of the eukaryotic cell. Nuclear extracts have been extensively used in different model organisms to identify and study protein function in nuclei. Drosophila embryos can be collected in large quantities and have been the source of choice for the production of protein nuclear extracts. However, most of Drosophila in vivo studies on protein function are conducted in larval tissues. Here we report a new method to produce highly stable large-scale protein nuclear extracts from whole Drosophila larvae that are suited for a variety of biochemical analyses.

La Rocca, G., Burgio, G., Corona, D.F. (2007). A protein nuclear extract from D. melanogaster larval tissue. FLY, 1(6), 343-345.

A protein nuclear extract from D. melanogaster larval tissue

BURGIO, Giosalba;CORONA, Davide
2007-01-01

Abstract

Preparation of protein nuclear extracts is often the first step to study in vitro biological processes occurring in the nucleus of the eukaryotic cell. Nuclear extracts have been extensively used in different model organisms to identify and study protein function in nuclei. Drosophila embryos can be collected in large quantities and have been the source of choice for the production of protein nuclear extracts. However, most of Drosophila in vivo studies on protein function are conducted in larval tissues. Here we report a new method to produce highly stable large-scale protein nuclear extracts from whole Drosophila larvae that are suited for a variety of biochemical analyses.
Settore BIO/10 - Biochimica
FLY
La Rocca, G., Burgio, G., Corona, D.F. (2007). A protein nuclear extract from D. melanogaster larval tissue. FLY, 1(6), 343-345.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/65609
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