A scalable, single-step, synthetic approach for the manufacture of biocompatible, functionalized micro- and nanogels is presented. In particular, poly(N-vinyl pyrrolidone)-grafted-(aminopropyl)methacrylamide microgels and nanogels were generated through e-beam irradiation of PVP aqueous solutions in the presence of a primary amino-group-carrying monomer. Particles with different hydrodynamic diameters and surface charge densities were obtained at the variance of the irradiation conditions. Chemical structure was investigated by different spectroscopic techniques. Fluorescent variants were generated through fluorescein isothiocyanate attachment to the primary amino groups grafted to PVP, to both quantify the available functional groups for bioconjugation and follow nanogels localization in cell cultures. Finally, a model protein, bovine serum albumin, was conjugated to the nanogels to demonstrate the attachment of biologically relevant molecules for targeting purposes in drug delivery. The described approach provides a novel strategy to fabricate biohybrid nanogels with a very promising potential in nanomedicine.

Dispenza, C., Sabatino, M.A., Grimaldi, N., Bulone, D., Bondì, M.L., Casaletto, M.P., et al. (2012). Minimalism in Radiation Synthesis of Biomedical Functional Nanogels. BIOMACROMOLECULES, 13(6), 1805-1817 [10.1021/bm3003144].

Minimalism in Radiation Synthesis of Biomedical Functional Nanogels

DISPENZA, Clelia;SABATINO, Maria Antonietta;GRIMALDI, Natascia;RIGOGLIUSO, Salvatrice;ADAMO, Giorgia;GHERSI, Giulio
2012-01-01

Abstract

A scalable, single-step, synthetic approach for the manufacture of biocompatible, functionalized micro- and nanogels is presented. In particular, poly(N-vinyl pyrrolidone)-grafted-(aminopropyl)methacrylamide microgels and nanogels were generated through e-beam irradiation of PVP aqueous solutions in the presence of a primary amino-group-carrying monomer. Particles with different hydrodynamic diameters and surface charge densities were obtained at the variance of the irradiation conditions. Chemical structure was investigated by different spectroscopic techniques. Fluorescent variants were generated through fluorescein isothiocyanate attachment to the primary amino groups grafted to PVP, to both quantify the available functional groups for bioconjugation and follow nanogels localization in cell cultures. Finally, a model protein, bovine serum albumin, was conjugated to the nanogels to demonstrate the attachment of biologically relevant molecules for targeting purposes in drug delivery. The described approach provides a novel strategy to fabricate biohybrid nanogels with a very promising potential in nanomedicine.
Settore CHIM/07 - Fondamenti Chimici Delle Tecnologie
Dispenza, C., Sabatino, M.A., Grimaldi, N., Bulone, D., Bondì, M.L., Casaletto, M.P., et al. (2012). Minimalism in Radiation Synthesis of Biomedical Functional Nanogels. BIOMACROMOLECULES, 13(6), 1805-1817 [10.1021/bm3003144].
File in questo prodotto:
File Dimensione Formato  
Minimalism in Radiation Synthesis of Biomedical Functional.pdf

accesso aperto

Dimensione 3 MB
Formato Adobe PDF
3 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/65346
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 42
  • ???jsp.display-item.citation.isi??? 39
social impact