Collagens are highly preserved proteins in invertebrates and vertebrates. To identify the collagens in urochordates, the total RNA extracted from the pharynx of the ascidian Ciona intestinalis was hybridized with a heterologous probe specific for the echinoderm Paracentrotus lividus fibrillar type I-like larval collagen. Using this probe, two main bands (i.e. 6 and 2.8 kb mRNA) were observed on Northern blot hybridization. The cDNA library prepared from poly(A) +RNA extracted from pharyngeal tissue was screened and a cDNA that specifies a type IX-like collagen was identified. This molecule presents a conceptual open reading frame for a protein containing 734 amino acids. In particular, we showed a 1a chain type IX-like collagen characterized by three short triple-helical domains interspersed with four non-triple-helical sequences, with structural features of fibril-associated collagens with interrupted triple-helices (FACIT) collagens. Northern blot hybridizations indicate a 2.8 kb transcript size. Sequence comparison indicated homology (47.64%, 48.95%) between the type IX-like collagen of C. intestinalis and mouse and human type IX collagen. In situ hybridization of tunic and pharynx tissues shows the presence of transcripts in connective tissue cells.
|Data di pubblicazione:||2002|
|Titolo:||Cloning and expression of a type IX-like collagen in tissues of the ascidian Ciona intestinalis|
|Autori:||Vizzini, A; Arizza, V; Cervello, M; Cammarata, M; Gambino, R; Parrinello, N|
|Tipologia:||Articolo su rivista|
|Citazione:||Vizzini, A., Arizza, V., Cervello, M., Cammarata, M., Gambino, R., & Parrinello, N. (2002). Cloning and expression of a type IX-like collagen in tissues of the ascidian Ciona intestinalis. BIOCHIMICA ET BIOPHYSICA ACTA, N. GENE STRUCTURE AND EXPRESSION, 1577, 38-44.|
|Tipo:||Articolo in rivista|
|Appare nelle tipologie:||01 - Articolo su rivista|