Actinomycetes are an important source of biologically active compounds, like antibiotics, antitumor agents, and immuno-suppressors. Genome sequencing is revealing that this class of microorganisms has larger genomes relative to other bacteria, and uses a considerable fraction of its coding capacity (5–10%) for the production of mostly cryptic secondary metabolites. To access actinomycetes biosynthetic capabilities or to improve the pharmacokinetic properties and production yields of these chemically complex compounds, genetic manipulation of the producer strains can be performed. Heterologous expression in amenable hosts can be useful to exploit and to explore the genetic potential of actinomycetes and not cultivable but interesting bacteria. Artificial chromosomes that can be stably integrated into the Streptomyces genome were constructed and demonstrated to be effective for transferring entire biosynthetic gene clusters from intractable actinomycetes into more suitable hosts. In this review, the construction of several shuttle Escherichia coli-Streptomyces artificial chromosomes is discussed together with old and new strategies applied to improve heterologous production of secondary metabolites.

Alduina, R., & Gallo, G. (2012). Artificial chromosomes to explore and exploit biosynthetic capabilities of actinomycetes. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2012 [10.1155/2012/462049].

Artificial chromosomes to explore and exploit biosynthetic capabilities of actinomycetes

ALDUINA, Rosa;GALLO, Giuseppe
2012

Abstract

Actinomycetes are an important source of biologically active compounds, like antibiotics, antitumor agents, and immuno-suppressors. Genome sequencing is revealing that this class of microorganisms has larger genomes relative to other bacteria, and uses a considerable fraction of its coding capacity (5–10%) for the production of mostly cryptic secondary metabolites. To access actinomycetes biosynthetic capabilities or to improve the pharmacokinetic properties and production yields of these chemically complex compounds, genetic manipulation of the producer strains can be performed. Heterologous expression in amenable hosts can be useful to exploit and to explore the genetic potential of actinomycetes and not cultivable but interesting bacteria. Artificial chromosomes that can be stably integrated into the Streptomyces genome were constructed and demonstrated to be effective for transferring entire biosynthetic gene clusters from intractable actinomycetes into more suitable hosts. In this review, the construction of several shuttle Escherichia coli-Streptomyces artificial chromosomes is discussed together with old and new strategies applied to improve heterologous production of secondary metabolites.
Settore BIO/19 - Microbiologia Generale
Alduina, R., & Gallo, G. (2012). Artificial chromosomes to explore and exploit biosynthetic capabilities of actinomycetes. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2012 [10.1155/2012/462049].
File in questo prodotto:
File Dimensione Formato  
JBB 2012.pdf

Solo gestori archvio

Dimensione 645.92 kB
Formato Adobe PDF
645.92 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/63845
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 19
  • ???jsp.display-item.citation.isi??? 14
social impact