Within a study focused on Sinapis pubescens subsp. pubescens wild from Sicily (Italy), an edible species still unexplored, our earlier published work has demonstrated good in vitro antioxidant properties for the flower and leaf hydroalcoholic extracts, exhibiting quite different qualitative-quantitative phenolic profiles. Herein, further research was designed to elucidate the role played by phenolic compounds in the different antioxidant mechanisms highlighted for the extracts. To achieve this goal, the crude extracts were subjected to liquid-liquid partitioning with solvents of increasing polarity; then, the fractions were investigated for their antioxidant properties using different in vitro assays. For both flowers and leaves, the ethyl acetate fractions exhibited the best activity in DPPH and reducing power assays, followed by n-butanol. The total phenolic content determination indicated these fractions as the phenolic-rich ones, which were characterized by HPLC-PDA/ESI-MS analysis. Conversely, the phenolic-rich fractions did not show any chelating activity, which was highlighted for the more hydrophobic ones.

Maria Fernanda Taviano, Paola Arena, Federica Davì, Emilia Cavò, Vivienne Spadaro, Francesco Maria Raimondo, et al. (2024). Contribution of Phenolic Compounds to the Antioxidant Activity of Leaf and Flower Extracts of Sinapis pubescens L. subsp. pubescens (Brassicaceae). CHEMISTRY & BIODIVERSITY [10.1002/cbdv.202400272].

Contribution of Phenolic Compounds to the Antioxidant Activity of Leaf and Flower Extracts of Sinapis pubescens L. subsp. pubescens (Brassicaceae)

Vivienne Spadaro;Francesco Maria Raimondo;
2024-01-01

Abstract

Within a study focused on Sinapis pubescens subsp. pubescens wild from Sicily (Italy), an edible species still unexplored, our earlier published work has demonstrated good in vitro antioxidant properties for the flower and leaf hydroalcoholic extracts, exhibiting quite different qualitative-quantitative phenolic profiles. Herein, further research was designed to elucidate the role played by phenolic compounds in the different antioxidant mechanisms highlighted for the extracts. To achieve this goal, the crude extracts were subjected to liquid-liquid partitioning with solvents of increasing polarity; then, the fractions were investigated for their antioxidant properties using different in vitro assays. For both flowers and leaves, the ethyl acetate fractions exhibited the best activity in DPPH and reducing power assays, followed by n-butanol. The total phenolic content determination indicated these fractions as the phenolic-rich ones, which were characterized by HPLC-PDA/ESI-MS analysis. Conversely, the phenolic-rich fractions did not show any chelating activity, which was highlighted for the more hydrophobic ones.
2024
Settore BIO/02 - Botanica Sistematica
Settore BIO/15 - Biologia Farmaceutica
Maria Fernanda Taviano, Paola Arena, Federica Davì, Emilia Cavò, Vivienne Spadaro, Francesco Maria Raimondo, et al. (2024). Contribution of Phenolic Compounds to the Antioxidant Activity of Leaf and Flower Extracts of Sinapis pubescens L. subsp. pubescens (Brassicaceae). CHEMISTRY & BIODIVERSITY [10.1002/cbdv.202400272].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/636313
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